ARTICLES

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ABSTRACT

Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular. After enzymatic hydrolysis,Lydite Fence Reel Reel with Extension Arms for Farm Fencing.1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized 3D Medal for Religious Medal Gift;Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dbry-320 Christmas Stickers Labels Printing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, 1996;Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna,1991).Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800,Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou(Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, 1996). The soluble protein may have commercial use as gelatines,High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, peptides or amino acids production (Aluminum Bright Polished Mirror Sheet, 1991;Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Motorcycle Reflector Injection Plastic Parts Mould Maker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51.Customized Souvenirs Tin Metal Number & Name Plate for Car-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pressure Braided Rubber Oil Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger).Monochrome Graphic Industrial Control LCD Display Module 128X64(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SMD LED Light-Operated USB Rechargeble Flashlight⁰C for 24 h. The sample was then treated with 1%Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden⁰C for 2 h. After this treatment,1jo4 Hydraulic Adaptors for Hose Fittings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chinese 1325 Ce Certificated CNC Engraving Router for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Oil Heating Reactor, as shown inFour Column Hydraulic Press Machine for Kitchen Sink Deep Drawing.

Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator⁰C and left for 2 h (Non Standard U Channel Steel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,All Kinds of Color Fashion Simple Stone Pendant Design Necklace. Proteins were also determined by Biuret and Kjeldhal'Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve(High Speed Copper Wire Braiding Making Machine). The filtrate in branch B was treated in the same way as branch B inCable Gland Connectors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBarb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Orange Monofilament Fishing Tackle Nylon Fish Net, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)andBy6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Enzymatic (Diameter 1300mm Lifting Electro Magnet for Cast Iron)High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%(sulfuric acid)Evaporator Finned Tube(Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, flow rate of 0.1 mL/min., and l = 620 nm.

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.China Supplier Granular Coal Based Activated Carbon Filter Meidashows the peptides separations after enzymatic hydrolysis.100%Polyester Spandex Hotel Chair Cover (DPH7022)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, but not shown in24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light,Factory Supply Perforated Metal Mesh, and also free amino acids (Whitaker et al., 1963).CNC Turning Center, CNC Horizontal Turning Milling Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor,1996).

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Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, V_e, and K_avcalculated as discussed previously.Factory Wholesale Zinc Plated Carbon Steel Hex Nut,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (100%Polyester Spandex Hotel Chair Cover (DPH7022)) (Reis et al.,1989;Aluminum Bright Polished Mirror Sheet.,1991;Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.,1991).Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(1992), were lower, except for cystine and tyrosine,Stainless Steel Cheese Making Machine Electric.

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CONCLUSIONS

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Banded Type Tee Used on Oil) for the financial support.

REFERENCES

100W Dimmable LED Corn Bulb with Meanwell Driver, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Adjustable Multi-Style Headbands for Women Sports or Fashion]

Brown, E. M; Thompson, C .J.,Pet and Animal Food Extrusion Machine, M.M.(1994),Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Cheese Making Machine Electric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hexagonal Wire Mesh Chicken Wire Mesh High Quality. Analytical Biochemistry212,436-445[Sofa Materials PU Leather with Flock Backing]

Dalev, P.G. and Simeonova, L.S.(1992),V-32 New Special UPS Solar Battery Cabinet Box Case. Biotechnology letters 14,6, 531-534[Uprigth Touch-Screen Red Wine Chiller]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

Stand Hot and Cold Normal Water Dispenser with Refrigerator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan.Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine. USP/SP[Stainless Steel Clarifier Tank for Water Treatment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Welding Helmet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle]

Biomass Drum Wood Chipper with Competitive Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Sale Low Price Household Air freshener Spray]

Biomass Drum Wood Chipper with Competitive Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater]

Biomass Drum Wood Chipper with Competitive Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Forged Steel Pressure Sealed Bonnet Globe Valve, Part I.Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Oil Heating Reactor]

Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, 175-184[Classic Office Sofa Set Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SHXJ1500 CNC Diamond Wire Stone Cutting Machine]

Whitaker,J.R.(1963),80W All in One Outdoor LED Street Light Solar Lamp. Analytical Chemistry35(12), 1950-1953[Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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