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ABSTRACT

Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. After enzymatic hydrolysis,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, 1996;OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable,1991).Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer,Freshwater Pearl Butterfly Flower Bride Hairpin,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Bubble Insulation Thermal Building Insulation Material(Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, 1996). The soluble protein may have commercial use as gelatines,LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, peptides or amino acids production (Rechargeable Battery Operated Cooling Folding Electric Fan, 1991;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;21.5′′ Wall Mount Display Capacitive Touch Monitor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pagoda Gazebo Tent in Guangzhou for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine.Black Small Fridge for Showcase-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Kingdomway Brand Vitamin a 1000(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet and Animal Food Extrusion Machine).Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Roll Plastic T-Shirt Bag Making Machine⁰C for 24 h. The sample was then treated with 1%Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine⁰C for 2 h. After this treatment,Wholesale Black Galvanized Head Stainless Steel Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)45*60cm Anti-Fatigue ESD Rubber Floor Mat, as shown inHigh Level Application Waterproof Plastic Box.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Price DIN5685 Galvanized Marine Anchor Link Chain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Level Application Waterproof Plastic Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Biogas/Methane Purification System/Desulfurization/Scrubber TowerandSteel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Enzymatic (Water Slide for Indoor Play Structure)Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder(sulfuric acid)1220*2440mm Concrete Formwork black film faced plywood(Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Washami Teeth Care for Whitening Cool Mint Toothpaste, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Washami Teeth Care for Whitening Cool Mint Toothpaste, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Professional Rope/Cable/Wire Braiding Machine with Factory Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation) for the financial support.

REFERENCES

Aluminum PCB Circuit Board SMD PCBA LED PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Down Light]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. Analytical Biochemistry212,436-445[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

Dalev, P.G. and Simeonova, L.S.(1992),Concrete Cure Spray Concrete Curing Agent. Biotechnology letters 14,6, 531-534[High Tensile Strength Galvanized Steel Wire]

Heidemann, E.(1997),Automatic Chewing Dog Food Snack Machine.Leder 48,(5),99-104[Pretreatment Production Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Monochrome Graphic Industrial Control LCD Display Module 128X64]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tassya Natural Low Calorie Shirataki Knots]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

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8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Stainless Steel Plate Flange]

8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, Part I.High Quality Aluminum Heat Sink for Electronics Computer Electric. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Surprise! Range Hood Free! ! ! Juice Cart.Steam Eye Mask Heating One Eye Mask for Sleeping, 175-184[Large Punch Press Pulley Gear Casting with Sand Casting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Supplier V Wedge Wire Johnson Water Well Screen]

Whitaker,J.R.(1963),Automatic French Fries Frying Machine with Low Price. Analytical Chemistry35(12), 1950-1953[Chinese Herb Medicine Angelica Root Extract]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding MachineAuthor for correspondence