ARTICLES

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ABSTRACT

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. After enzymatic hydrolysis,Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery.CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi-Functional Backpack Manual 52cc Brush Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm,1991).White Cotton Satin Hotel Duvet Cover (DPFB8055). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Cleaning Brush ESD Anti Static Brush ESD Brush Black,PAC /Poly Aluminium Chloride for Water Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Sheet Shearing Machine(Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996). The soluble protein may have commercial use as gelatines,Electrical PV DC Solar Cable, peptides or amino acids production (Tomato Bubble and High Pressure Washing Machine, 1991;Upholstery Ribbon Christmas Tape, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2+1 Flavors Soft Ice Cream Machine with Pre-Cooling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse.Best Sale Low Price Household Air freshener Spray-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Supplied High Strength Marine Anchor Chain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Women Fashion Winter V Neck Long Sleeve Clothes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part).Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D CNC Router for Wood Cutting Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Screens / Wedge Wire Filter Element⁰C for 2 h. After this treatment,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EPS Foam Sheet Extrusion Machine for Fast Food Box Making's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, as shown inFactory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Low Price Top Selling Fashion Summer Boy Sandals. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEasy Operational Cup Cake Filling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Folding Play Mat Crawl Play Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)) for the financial support.

REFERENCES

Manufacture Best Choice Liquid Flocculant for Ink Removal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Cast Cookware Frying Pans]

Brown, E. M; Thompson, C .J.,Hot Dipped Galvanized Oval Wire Brazil, M.M.(1994),Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pipe Thermostat with Sensor Ce Certificate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film. Analytical Biochemistry212,436-445[Gold Star Square Glass Candle Holder for Wedding Decoration]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sophisticated Durable Membrane Panel/Panel with 3m Glue: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Strength GRP Cooling Towers for Mechanical Cooling]

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Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps]

Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, Part I.Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dxz Limit Switch Tower Crane Spare Parts]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Chewing Dog Food Snack Machine]

Whitaker,J.R.(1963),2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. Analytical Chemistry35(12), 1950-1953[Orange Monofilament Fishing Tackle Nylon Fish Net]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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