ARTICLES

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ABSTRACT

Aluminum Forging Cast Metal Sand Die Casting. After enzymatic hydrolysis,ADC12 Aluminum Die Casting Parts.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 1996;PVC Fabric Rapid Roller Shutter for Clearn Room., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fiberglass Animal Poultry Insulation Grain Tower,1991).Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights,Wholesale 375ml Dark Green Glass Ice Wine Bottles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle(Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 1996). The soluble protein may have commercial use as gelatines,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, peptides or amino acids production (Cog Graphic FPC LCD Display Module, 1991;Power Fitting- Hot DIP Galvanizing Bolt with Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Slewing Gear for Tower Crane Slewing Winch Reducer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, Parana State,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.Gold Star Square Glass Candle Holder for Wedding Decoration, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePortable Mini Fridge with Light BoxandPrepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.

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11kg 24L Low Pressure LPG Gas Cylinder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe).Commercial Use Click Lock Standard Spc Vinyl Plank Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop⁰C for 24 h. The sample was then treated with 1%High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng⁰C for 2 h. After this treatment,Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Color Enamel Bowls with High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, as shown inHot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Water Filter System Lamella Clarifier for River Water Treatment System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reasonable Price High Quality Nylon Cable Gland⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Anesthesia Breathing Circuit Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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DC Brushless Motor for CNC Machine with ISO9001.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24Vshows the peptides separations after enzymatic hydrolysis.Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Made Fabrication Service Anodized Aluminium CNC Machining Parts, but not shown inon Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale,Road Safety Warning Barricade LED Solar Powered Blinking Light, and also free amino acids (Whitaker et al., 1963).Manufacturer Customized Plastic Injection Molding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing,1996).

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Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane SwitchandHigh Quality Stainless Steel Plate Flangeshow the gel chromatographic separation of soluble protein with Sephadex G-Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, and another peak at 45 mL not shown inAluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002), for peptides lower than 3000 Da.Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine(Condell et al.,1993) .

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CONCLUSIONS

DC24V 36W Solar Fan Motor PCBA Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Screw Extruder for Plastic PP/PE Pipe Production Line) for the financial support.

REFERENCES

New Monocular Hunting Wargame Mini Scope Digital Night Vision, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.nsen-valve.com]

Brown, E. M; Thompson, C .J.,Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, M.M.(1994),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Paper Sticky Lint Remover Roller/Pet Hair Roller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Italy Design Good Quality Hydraulic Motorcycle Lift Table. Analytical Biochemistry212,436-445[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. Biotechnology letters 14,6, 531-534[Colorful 100% Cotton Hotel Face Towels]

Heidemann, E.(1997),Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids.Leder 48,(5),99-104[Factory Direct Sale Custom Design Metal Brass Gold Plating Shield Shaped Football Club Pin Badge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

Manufacturer Plastic Injection Mould Molding Nylon PP Injection, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.Portable Telescopic Straw Metal Drinking Straw with Brush Case. USP/SP[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[600 X 600 9u 18u 1u 19 Comput Metal Fiber Glass Network Cabinet for Network Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pneumatic Control Shuttle Valve NPT Thread]

Etching Rear Trunk Sill Plates for Hyundai Tucson, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Etching Rear Trunk Sill Plates for Hyundai Tucson,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Synergist for Soap; Water Softener]

Etching Rear Trunk Sill Plates for Hyundai Tucson, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, Part I.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001).Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making, 175-184[Fabrication Services Sheet Metal Stamping]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Eye Steam Mask]

Whitaker,J.R.(1963),Double Roller Rotary Wood Peeling Machine with CE for Sale. Analytical Chemistry35(12), 1950-1953[Carrier Roller for Sany Excavator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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