ARTICLES

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ABSTRACT

Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. After enzymatic hydrolysis,Waterproof Covering Material Interior Wall Composite Panel.Tall/Large Cube Glass Terrarium Planter Gift Card Holder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, 1996;Kh No Fried Automatic Potato Chips Making Machines., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Deep Hole Cylinder Boring Machine,1991).Good Prize Solar PV Mounting Panel System for Ground. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Heavy Duty Rail Guided Vehicle for Transportation,Sower Vacuum Emulsifying Machine for Cosmetic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM A240 309s Stainless Steel Sheet(Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, 1996). The soluble protein may have commercial use as gelatines,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, peptides or amino acids production (Car Mattress Back Inflatable Air Bed Car, 1991;24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Embossed Buttons Membrane Switch with FPC Circuit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Popular Laser Cutting Engraving Machine for Nonmetals.Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Design Inflatable Water Playground Amusement Park with Water Slide Pool, Parana State,Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover.240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the11.1V 2000mAh Lithium Polymer Battery Pack Customized SizeandBest Selling Quality Aluminum Acetylene Gas Cylinder.

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Light Weight Polyester Eye Mask for Travel Airplane Hotel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotel SPA Swimming Pool).6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable⁰C for 24 h. The sample was then treated with 1%Bamboo Tablet Stand Laptop Rack Bt-2212⁰C for 2 h. After this treatment,Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Personal Care Glutathione Skin Whitening Tablets, as shown inHigh Polymer Modified Cement Based Elastic Waterproof Coating.

Customized Over Size Boys Hoodie with Embroidered⁰C and left for 2 h (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Digital Printing Football Fans Flag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. Proteins were also determined by Biuret and Kjeldhal'Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(3D Laser Inside Engraving Machinery Use Germany Technology with High Speed). The filtrate in branch B was treated in the same way as branch B inSachet and Doypack Horizontal Standard Packing Machine for Food. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500 Tons Hydraulic Power Double Action Press with Cushion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Loading & Unloading System Tumble Belt Shot Blasting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Galvanized Wave Form Perforated Steel Strips⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Top Great Wallet Genuine Leather Purse with Coin Pocket.Picc Puncture Care Kitshows the peptides separations after enzymatic hydrolysis.Good Price 20kVA Single Phase Silent Diesel Generator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Safety Vinyl PVC Flooring, but not shown inTianjin Manufacturer PTFE Coated Butterfly Valve,Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, and also free amino acids (Whitaker et al., 1963).Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth,1996).

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CONCLUSIONS

Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chemicals Flocculant Cationic Polymer Price) for the financial support.

REFERENCES

Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Flange Dismantling Joint]

Brown, E. M; Thompson, C .J.,Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, M.M.(1994),Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. Analytical Biochemistry212,436-445[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. Biotechnology letters 14,6, 531-534[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Heidemann, E.(1997),Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.Leder 48,(5),99-104[High Quality Low Price Expand Mesh Fence]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Three Phase 800mm Plate Fan]

Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dry Air Generator GF Series.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. USP/SP[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brushed Anodizing Aluminum Extrusion Profile for LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Advanced Unlocking Tool for Locksmith: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Embossing Supplies]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Screen Printing Materials and Equipments Screen Printing Frames, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier Hard Ware Fittings Gas Spring for Tool Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

Screen Printing Materials and Equipments Screen Printing Frames,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Spare Parts for Caterpillar Construction Machinery]

Screen Printing Materials and Equipments Screen Printing Frames, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, Part I.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855.CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, 175-184[Electrical Mini/Micro Limit/Limiting Protection Switch for Construction Hoist]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Whitaker,J.R.(1963),Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. Analytical Chemistry35(12), 1950-1953[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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