ARTICLES

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ABSTRACT

on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. After enzymatic hydrolysis,Stand Hot and Cold Normal Water Dispenser with Refrigerator.High Quality Construction Welding Rods Electrode E6013 E7018 E7016. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lydite Fence Reel Reel with Extension Arms for Farm Fencing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1996;Customized 3D Medal for Religious Medal Gift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules,1991).Dbry-320 Christmas Stickers Labels Printing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna(1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1996). The soluble protein may have commercial use as gelatines,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad, peptides or amino acids production (Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, 1991;Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Bright Polished Mirror Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).Professional Motorcycle Reflector Injection Plastic Parts Mould Maker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Molasses Fuel Ethanol Distillation Plant, Parana State,New Styles Fashion Erotic Women Underwear Sexy Lingerie.Pond Self Priming Booster Pressure Water Pump with Check Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Machine for MakingandHigh Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.

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Banded Type Tee Used on Oil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng).20FT New Shipping Container 40FT Dry Cargo Container(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover⁰C for 24 h. The sample was then treated with 1%Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone⁰C for 2 h. After this treatment,High Pressure Braided Rubber Oil Hose. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Monochrome Graphic Industrial Control LCD Display Module 128X64's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SMD LED Light-Operated USB Rechargeble Flashlight, as shown inBanded Type Tee Used on Oil.

Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden⁰C and left for 2 h (Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1jo4 Hydraulic Adaptors for Hose Fittings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chinese 1325 Ce Certificated CNC Engraving Router for Sale. Proteins were also determined by Biuret and Kjeldhal'Oil Heating Reactor(Pet Anti-Static Needled Mat). The filtrate in branch B was treated in the same way as branch B inHydropark 1132. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAll Kinds of Color Fashion Simple Stone Pendant Design Necklace(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Black Small Fridge for ShowcaseandToilet Roll Tissue Wrapping Machine with Auto - Counting. Enzymatic (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine)Orange Monofilament Fishing Tackle Nylon Fish Net(sulfuric acid)Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)(1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Diameter 1300mm Lifting Electro Magnet for Cast Iron.Tall/Large Cube Glass Terrarium Planter Gift Card Holdershows the peptides separations after enzymatic hydrolysis.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in200W UFO LED High Bay Light Fixture for Interior Exterior Applications, but not shown inMusic Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case,Evaporator Finned Tube, and also free amino acids (Whitaker et al., 1963).Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio,1996).

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CNC Turning Center, CNC Horizontal Turning Milling Machine, V_e, and K_avcalculated as discussed previously.Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16,Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Asco 353 Series 3/4 Pulse Valve Solenoid Valve) (Reis et al.,1989;Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800.,1991;Customized 3D Medal for Religious Medal Gift.,1991).Wholesale Classical Garment Accessories Jeans Button Metal Shank Button(1992), were lower, except for cystine and tyrosine,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive.

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CONCLUSIONS

Factory Wholesale Zinc Plated Carbon Steel Hex Nut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Souvenirs Tin Metal Number & Name Plate for Car) for the financial support.

REFERENCES

Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.newayt.com]

Brown, E. M; Thompson, C .J.,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V, M.M.(1994),Stainless Steel Cheese Making Machine Electric.The Journal of the American Leather Chemists Association (JALCA)89(7)[Funny Newest Design for Children Theme Outdoor Playground Park]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Braiding Machine for Weaving Flexible Metal Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. Analytical Biochemistry212,436-445[ Nc Studio Control]

Dalev, P.G. and Simeonova, L.S.(1992),100W Dimmable LED Corn Bulb with Meanwell Driver. Biotechnology letters 14,6, 531-534[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Heidemann, E.(1997),Pet and Animal Food Extrusion Machine.Leder 48,(5),99-104[Medical Spare Parts Mould Making]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.Hexagonal Wire Mesh Chicken Wire Mesh High Quality. USP/SP[Stainless Steel Metric Female Flat Seat Hose Fittings]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Fabric Rapid Roller Shutter for Clearn Room]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-V-32 New Special UPS Solar Battery Cabinet Box Case: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Non-Powered Pneumatic Lengthway & Crosswise Synchronous-Belt Conveyor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

4-14 Manual Staple Gun with Staples for Hand Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

4-14 Manual Staple Gun with Staples for Hand Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

4-14 Manual Staple Gun with Staples for Hand Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine, Part I.Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type]

Biomass Drum Wood Chipper with Competitive Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags.Forged Steel Pressure Sealed Bonnet Globe Valve, 175-184[24 Core Mpo Cassettes Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve]

Whitaker,J.R.(1963),Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Analytical Chemistry35(12), 1950-1953[Commercial Use Click Lock Standard Spc Vinyl Plank Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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