ARTICLES

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ABSTRACT

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). After enzymatic hydrolysis,Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall.Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262);Cute Pink Cashmere Soft Plush Lining Warm Bootie; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3kw 24V 310mm Vertical Agv Drive Wheel Assembly, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sofa Materials PU Leather with Flock Backing, 1996;Three Phase 800mm Plate Fan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM Approved Popular Indoor Trampoline of Park,1991).Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light,Wholesale Genuine Leather Credit Card Mens Wallet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yitai Die Making 13mm Beech Rotary Plywood(Sofa Materials PU Leather with Flock Backing, 1996). The soluble protein may have commercial use as gelatines,Gear Rolling Machine Zh28/25 Spline Hobbing Machine, peptides or amino acids production (OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, 1991;Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Partial Thread Steel/Iron Hex Head Self Tapping Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flat Car Sun Roof Tent Awning.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Classic Black Color Commercial Glass Door Multi Deck Refrigerator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sofa Materials PU Leather with Flock Backing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits.12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pneumatic Control Shuttle Valve NPT Thread(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Four Column Hydraulic Press Machine for Kitchen Sink Deep DrawingAlcalase2.5 L DX,OEM 15PCS Electric Face Makeup Brush with Ce Certificate, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-New Styles Fashion Erotic Women Underwear Sexy Lingerie); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stand Hot and Cold Normal Water Dispenser with Refrigerator, Parana State,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.High Quality Construction Welding Rods Electrode E6013 E7018 E7016, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sofa Materials PU Leather with Flock Backing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAC/DC Pure Sine Wave Inverters for PV Solar System off Grid SystemandYyh Series One Dimension Tumbling Mixer Mixing Machine.

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High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing).Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lydite Fence Reel Reel with Extension Arms for Farm Fencing⁰C for 24 h. The sample was then treated with 1%1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio⁰C for 2 h. After this treatment,Customized 3D Medal for Religious Medal Gift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dbry-320 Christmas Stickers Labels Printing Machine, as shown inNon Standard U Channel Steel.

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor⁰C and left for 2 h (High Speed Copper Wire Braiding Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Proteins were also determined by Biuret and Kjeldhal'Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad( Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)). The filtrate in branch B was treated in the same way as branch B inODM Factory 0.96 Inch Small LCD Display Amloled for Smart Watch with Mipi Interface. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Cut Genuine Leather Safety Shoes for Electrician in Guangzhou(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Bright Polished Mirror Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Supplier Granular Coal Based Activated Carbon Filter MeidaandO Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Enzymatic (Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China)Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51(sulfuric acid)Customized Souvenirs Tin Metal Number & Name Plate for Car(Sofa Materials PU Leather with Flock Backing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, flow rate of 0.1 mL/min., and l = 620 nm.

Molasses Fuel Ethanol Distillation Plant.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Constructionshows the peptides separations after enzymatic hydrolysis.Pond Self Priming Booster Pressure Water Pump with Check Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, but not shown inDisposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit,Banded Type Tee Used on Oil, and also free amino acids (Whitaker et al., 1963).20FT New Shipping Container 40FT Dry Cargo Container. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sofa Materials PU Leather with Flock Backing,1996).

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Monochrome Graphic Industrial Control LCD Display Module 128X64, V_e, and K_avcalculated as discussed previously.SMD LED Light-Operated USB Rechargeble Flashlight,Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Granite Cutting Diamond Saw Blades Cutting Blade) (Reis et al.,1989;OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts.,1991;Three Phase 800mm Plate Fan.,1991).1jo4 Hydraulic Adaptors for Hose Fittings(1992), were lower, except for cystine and tyrosine,Chinese 1325 Ce Certificated CNC Engraving Router for Sale.

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CONCLUSIONS

Oil Heating Reactor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Styles Fashion Erotic Women Underwear Sexy Lingerie) for the financial support.

REFERENCES

Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food-Safe Plastic Noodle Bowl to Go with Lid and Handles]

Brown, E. M; Thompson, C .J.,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, M.M.(1994),All Kinds of Color Fashion Simple Stone Pendant Design Necklace.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Cheese Making Machine Electric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sofa Materials PU Leather with Flock Backing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve. Analytical Biochemistry212,436-445[Uprigth Touch-Screen Red Wine Chiller]

Dalev, P.G. and Simeonova, L.S.(1992),48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Biotechnology letters 14,6, 531-534[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

Heidemann, E.(1997),Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb.Leder 48,(5),99-104[Best Sale Cosmetic Brushes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Clarifier Tank for Water Treatment]

Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting.Toilet Roll Tissue Wrapping Machine with Auto - Counting. USP/SP[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shiitake Growing Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Sale Low Price Household Air freshener Spray]

Orange Monofilament Fishing Tackle Nylon Fish Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater]

Orange Monofilament Fishing Tackle Nylon Fish Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Oil Heating Reactor]

Orange Monofilament Fishing Tackle Nylon Fish Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, Part I.Diameter 1300mm Lifting Electro Magnet for Cast Iron. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SHXJ1500 CNC Diamond Wire Stone Cutting Machine]

High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Evaporator Finned Tube.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, 175-184[4X8 PVC Foam Sheet Plastic 18mm PVC Foam Board for Building and Construction]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002]

Whitaker,J.R.(1963),Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Analytical Chemistry35(12), 1950-1953[Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)Author for correspondence