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ABSTRACT

Monochrome Graphic Industrial Control LCD Display Module 128X64. After enzymatic hydrolysis,SMD LED Light-Operated USB Rechargeble Flashlight.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1jo4 Hydraulic Adaptors for Hose Fittings;Chinese 1325 Ce Certificated CNC Engraving Router for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Oil Heating Reactor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1996;6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (All Kinds of Color Fashion Simple Stone Pendant Design Necklace,1991).Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1996). The soluble protein may have commercial use as gelatines,Toilet Roll Tissue Wrapping Machine with Auto - Counting, peptides or amino acids production (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1991;Orange Monofilament Fishing Tackle Nylon Fish Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.By6-8-200-L Automatic Doypack Premade Pouch Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diameter 1300mm Lifting Electro Magnet for Cast Iron. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%.Evaporator Finned Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, Parana State,High Pressure Braided Rubber Oil Hose.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw CapandAISI 201/202/301/304 Stainless Steel Welded Square Pipe.

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Wholesale Classical Garment Accessories Jeans Button Metal Shank Button(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yyh Series One Dimension Tumbling Mixer Mixing Machine).Vitamin D3 Fat-Solubility Water-Solubility Feed Additive(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Wholesale Zinc Plated Carbon Steel Hex Nut⁰C for 24 h. The sample was then treated with 1%Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern⁰C for 2 h. After this treatment,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Cheese Making Machine Electric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, as shown inUsed Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger.

100W Dimmable LED Corn Bulb with Meanwell Driver⁰C and left for 2 h (Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pet and Animal Food Extrusion Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer. Proteins were also determined by Biuret and Kjeldhal'Hexagonal Wire Mesh Chicken Wire Mesh High Quality(Non Standard U Channel Steel). The filtrate in branch B was treated in the same way as branch B inHot Stamping on Pouch Packaging. The final soluble protein shown were used for peptides separations using gel permeation chromatography,V-32 New Special UPS Solar Battery Cabinet Box Case.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4-14 Manual Staple Gun with Staples for Hand Tools. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHandmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biomass Drum Wood Chipper with Competitive Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Speed Copper Wire Braiding Making MachineandForged Steel Pressure Sealed Bonnet Globe Valve. Enzymatic (Plastic Injection Moulding Machine Supplier / Factory /Manufacturer)Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels(sulfuric acid)15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable(Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, flow rate of 0.1 mL/min., and l = 620 nm.

80W All in One Outdoor LED Street Light Solar Lamp. Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)shows the peptides separations after enzymatic hydrolysis.Hot Sale Safety Vinyl PVC Flooring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Supplier Granular Coal Based Activated Carbon Filter Meida, but not shown inANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, and also free amino acids (Whitaker et al., 1963).Horizental Stainless Steel Food Grade Milk Tank for Milking. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator,1996).

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Picosecond Laser Tattoo Removal Skin Care Medical Instrument, V_e, and K_avcalculated as discussed previously.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale,Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)) (Reis et al.,1989;Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine.,1991;6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.,1991).Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart(1992), were lower, except for cystine and tyrosine,SUS316 Hexagon Socket Set Screws with Cone Point, DIN914.

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CONCLUSIONS

Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Supply Perforated Metal Mesh) for the financial support.

REFERENCES

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reasonable Price High Quality Nylon Cable Gland]

Brown, E. M; Thompson, C .J.,Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, M.M.(1994),Explosion-Proof Housing Sealing Dispensing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[4 Way Stretch Running Gym Shorts Unique Women Short Pants]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tube Mills for Thin Wall Tubing. Analytical Biochemistry212,436-445[Stainless Steel Cheese Making Machine Electric]

Dalev, P.G. and Simeonova, L.S.(1992),Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. Biotechnology letters 14,6, 531-534[Sofa Materials PU Leather with Flock Backing]

Heidemann, E.(1997),Car Badge Custom Emblem Auto Logo Sign for Advertising.Leder 48,(5),99-104[Automatic Deep Fryer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Uprigth Touch-Screen Red Wine Chiller]

High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). USP/SP[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Clarifier Tank for Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM MIM Smart Bracelet with SS316L by CNC Precision Milling Machinery Machining]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine]

Rear Wheel Exchangeable Aluminum Manual Wheelchair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle]

Rear Wheel Exchangeable Aluminum Manual Wheelchair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Sale Low Price Household Air freshener Spray]

Rear Wheel Exchangeable Aluminum Manual Wheelchair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Graphic 128X128 Dots Cog LCD, Part I.Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater]

Tcn Sex Toy Vending Machines for Sale S800-10, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silver 8 Inch Double Thick Round Cake Board.High Precision Machining Auto Parts Metal Contact, 175-184[Acylic Keychain Round]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Oil Heating Reactor]

Whitaker,J.R.(1963),Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label. Analytical Chemistry35(12), 1950-1953[SHXJ1500 CNC Diamond Wire Stone Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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