ARTICLES

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ABSTRACT

40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse. After enzymatic hydrolysis,Best Sale Low Price Household Air freshener Spray.Factory Supplied High Strength Marine Anchor Chain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Humidity Refrigeration Digital Controller Jsd-100+;Elegant Europe Dessign Pillow Top Mattress; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, 1996;Modern 4 Person Workstation with Computer Desk Modular Office Furniture., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill,1991).Women Fashion Winter V Neck Long Sleeve Clothes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line,3D CNC Router for Wood Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Screens / Wedge Wire Filter Element(Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, 1996). The soluble protein may have commercial use as gelatines,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, peptides or amino acids production (EPS Foam Sheet Extrusion Machine for Fast Food Box Making, 1991;Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine.High Quality Stainless Steel Plate Flange-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Low Price Top Selling Fashion Summer Boy Sandals(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pump Flower Sprinkler with Automatic Watering System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag).Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field⁰C for 24 h. The sample was then treated with 1%Low Carbon Heavy Galvanized Steel Wire⁰C for 2 h. After this treatment,Long Distance Measurement Power Laser Digital Meter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets, as shown inWholesale Menthol E Liquid E Juice for EGO E Cigarettes.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2ton R Type Electric Wire Rope Hoist with Single Speed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Body Infrared Burner Good Selling Gas Stove(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adhesive CAS 9003-35-4 Phenolic Resin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacture Best Choice Liquid Flocculant for Ink Removal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Foil PE Bottle Cap Induction Seal Liner.Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tagshows the peptides separations after enzymatic hydrolysis.Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCarboxy Terminal Collagen Crosslinks Elisa Kits, but not shown inPopular Custom School Uniform Woven Badge Patch with Merrow Border,Sophisticated Durable Membrane Panel/Panel with 3m Glue, and also free amino acids (Whitaker et al., 1963).Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts,1996).

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CONCLUSIONS

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Folding Play Mat Crawl Play Mat) for the financial support.

REFERENCES

3*19.8 NRA G2 AISI420 Needle Roller for Bearing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Li-Polymer/Pouch Type Lithium Ion Lithium Battery Cell 3.2V 20ah]

Brown, E. M; Thompson, C .J.,JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, M.M.(1994),3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad.The Journal of the American Leather Chemists Association (JALCA)89(7)[Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Connector Sleeve Soft Plastic Battery Terminal Covers. Analytical Biochemistry212,436-445[Surprise! Range Hood Free! ! ! Juice Cart]

Dalev, P.G. and Simeonova, L.S.(1992),Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book. Biotechnology letters 14,6, 531-534[Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop]

Heidemann, E.(1997),Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer.Leder 48,(5),99-104[PVC Laminated Gypsum Ceiling Tiles for Home Interior Wall Decoration Design]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine]

Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Portable Mini Fridge with Light Box.Agriculture Film for Corn Silage Wrap Baler. USP/SP[ Foldable Esg10310]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Decorative House Stone Coated Metal Roof Tile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Gabion Wire Mesh Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[UHP 400 Graphite Electrode for Steel Mill a Right Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Double Layer Filter USD in POY Machines-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Filter Strainer]

Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away, Part I.Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Graphite Ring Good Lubrication, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Tower 6-Axis Screwdriver Machine Robot Manufacturer.Mslqs-P Needle-Free Injector/Needleless Syringe Price, 175-184[Double Hex Bolt Factory & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

Whitaker,J.R.(1963),YAMAHA Yv100II Nozzle Horder Square Head Short Type. Analytical Chemistry35(12), 1950-1953[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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