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ABSTRACT

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. After enzymatic hydrolysis,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ACP Aluminium Cmposite Panel Cladding Extrusions, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, 1996;Wholesale Custom Mens Heated Down Jacket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rectification Triming Metallized Polyester Film Slitting Rewinding Machine,1991).Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Swim Shorts Grey Mens Board Shorts with Mesh Liner,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, 1996). The soluble protein may have commercial use as gelatines,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, peptides or amino acids production (Marine Mooring Hardware, Anchor Chain, 1991;Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.QJ-M Moving small manual brick making machine with low cost.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set.Braiding Machine for Weaving Flexible Metal Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CAT6A FTP Patch Panel (WD6A-012)⁰C and left for 2 h (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Proteins were also determined by Biuret and Kjeldhal'Famous Brand Luxury Packaging Box Gift Box(2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle). The filtrate in branch B was treated in the same way as branch B inComfortable Sportswear Colorful Sexy Cycling Bib Shorts for Women. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Qualified Medical Grade Thin Wall Stainless Steel Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Strength New 0.18mm Molybdenum Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

UF Membrane Aqu160 with Competitive Price and High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light) for the financial support.

REFERENCES

Atf-805 Auto Car Transmission System Flushing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2000X4500mm Ce Certified Industrial PVB Laminated Glass Machinery (SN-BGF2045)]

Brown, E. M; Thompson, C .J.,43 Glass Beads Cat Eye Aluminum Road Stud, M.M.(1994),Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041).The Journal of the American Leather Chemists Association (JALCA)89(7)[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 3FT 3000lm IP66 Approved]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision C45 Steel CNC Lathe Parts. Analytical Biochemistry212,436-445[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

Dalev, P.G. and Simeonova, L.S.(1992),I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. Biotechnology letters 14,6, 531-534[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Heidemann, E.(1997),CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.Leder 48,(5),99-104[Nonwoven Geomembrane]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1.Food Grade PTFE Coated Fiberglass Mesh Baking Sheet. USP/SP[Transparent Composite Fiberglass Roof Tiles]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beverage Cooling Cube Ice Making/Ice Maker Machinery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ with Handle Vegetable Oil Nonstick]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bespoke Precision High Purity Sintering Graphite Mould]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Printed 100% Polyester Tcheap Wholesale Blankets]

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Transparent Composite Fiberglass Roof Tiles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[150kg Per Hour Frozen French Fries Production Line]

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, Part I.Two Layer License Plates with Wide Border Embossed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)]

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Really Microfiber PVC Leather for Sofa Furniture.94V0 Circuit Board Mobile Phone PCB Motherboard, 175-184[4.4.2 Laminated Glass]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Borosilicate Glass Baking Dish Set Clear]

Whitaker,J.R.(1963),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. Analytical Chemistry35(12), 1950-1953[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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