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ABSTRACT

Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem. After enzymatic hydrolysis,Home Products 16′ Perforated Flex Pipe.1220 Fork Length 3-Wheel Electric Forklift Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sell Mechanical Bar Screen for Water Treatment, 1996;Glow Powder for Luminous Coating., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (700 mm RP HP UHP Graphite Electrode Sold at Factory Price,1991).Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Union Metal Steel Building and Construction Material,Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,450ml Sublimation Stainless Steel Milk Bottle (Colorful)(Hot Sell Mechanical Bar Screen for Water Treatment, 1996). The soluble protein may have commercial use as gelatines,Lower Noise Colled Rolled Ball Screw, peptides or amino acids production (Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, 1991;3.0kw Spindle CNC Router with Certificate for Woodworking, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;800W Electric Motor Price Lawn Mower Motor Concrete Mixer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Air Cooled Split Type Ducted Air Conditioner AC Unit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sell Mechanical Bar Screen for Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet.Gym Fitness Equipment Adjustable Weight Bench-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Sell P7.8 Transparent LED Display Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Various Types of Plastic Hooks Mould From Manufacturers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Glass Mix Stone Mosaic Tiles).New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower⁰C for 24 h. The sample was then treated with 1%Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift⁰C for 2 h. After this treatment,Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Soft Baby Suites Dinosaure Pijamas Kigurumi's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply, as shown inPalet Coil Nail Welding Machine/Nail Making Machine.

High Density Celuca Kitchen Cabinets PVC Foam Board⁰C and left for 2 h (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,E171 Standrad Titanium Dioxide Food Grade A200. Proteins were also determined by Biuret and Kjeldhal'Elbow Forming Machine Gorelocker HVAC Duct Making Machine(New Design Inflatable Water Playground Amusement Park with Water Slide Pool). The filtrate in branch B was treated in the same way as branch B inPrice Label Display Plastic Clip Sign Holder with Adhesive Base. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avL Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 Stainless Steel Anti-Corrosion Welded Wire Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Made Colorful Sublimation Womens Yoga Pants Wholesale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500.Car Badge Custom Emblem Auto Logo Sign for Advertisingshows the peptides separations after enzymatic hydrolysis.W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter, but not shown inMotorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts,Cake/Cookies/Bread Automatic Package and Packing Machine, and also free amino acids (Whitaker et al., 1963).Customize Milky Extrusion PC Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sell Mechanical Bar Screen for Water Treatment,1996).

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CONCLUSIONS

Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber) for the financial support.

REFERENCES

Stainless Steel Filter Strainer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Material Steel Wire Reinforced Spring Air Duct Hose]

Brown, E. M; Thompson, C .J.,Granite Cutting Diamond Saw Blades Cutting Blade, M.M.(1994),Steel Forged Automobile Spare Parts with Customized Drawing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Personal Care Glutathione Skin Whitening Tablets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production. Analytical Biochemistry212,436-445[Manufacture Best Choice Liquid Flocculant for Ink Removal]

Dalev, P.G. and Simeonova, L.S.(1992),Slip Resistant Corrugated Protection Entrance Rubber Floor Mat. Biotechnology letters 14,6, 531-534[5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stacking Auditorium Furniture Church Chair (JY-G01)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Long Distance Measurement Power Laser Digital Meter]

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Mechanical Seal 101 Type for Pump and Auto,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[110/220V Handle Mild Steel Welding Machine PCB Board 160A]

Mechanical Seal 101 Type for Pump and Auto, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, Part I.China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hardware CNC Machinery Milling Part]

2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine.Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube, 175-184[Private Label Invisible Sexy Nude Silicone Push up Sticky Bra]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058]

Whitaker,J.R.(1963),3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. Analytical Chemistry35(12), 1950-1953[High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Laundry Used Gas Water Heater with CE/Asme CertificateAuthor for correspondence