ARTICLES

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ABSTRACT

Mold Injection Molding Plastic Products White PC Housing. After enzymatic hydrolysis,Sw Type BOPP Packaging Adhesive Tape Coating Machine.Stainless Steel Metric Female Flat Seat Hose Fittings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi Color Spade Made of Steel and Panting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, 1996;Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Condition Hot Sale Type Air Cooled Ice Cube Maker,1991).Stainless Steel Hinge Pin, Locking Hinge Pin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4 Way Stretch Running Gym Shorts Unique Women Short Pants,VHDCI Connector, 100p, Solder 0.8mm Pitch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube(Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, 1996). The soluble protein may have commercial use as gelatines,Must 4kw 2HP Pump Power Inverter Ags Bts Available, peptides or amino acids production (K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, 1991;Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100W 120W 150W Outdoor Solar LED Street Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.Economic Full Automated SMT Production Line PCB Production Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, Parana State,Square Decorative Stainless Steel Crimped Wire Mesh.Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Machine Cube Ice Machine for Commercial UseandHigh Quality Powder and Wax Brush with Wooden Handle.

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2.0mm Yellow Markers for Fiber Optic Multi Patch Cord(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FRP Water Tank GRP Fishing Tanks From Factory Supply).Chinese Galvanized Rectangular Tube for Buildings Materials(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Customized Chilled Cast Iron Roll⁰C for 24 h. The sample was then treated with 1%IMTEK High Precision and Rigidity Linear Guides for CNC machinery⁰C for 2 h. After this treatment,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yashi Touch Screen Smart Interactive Whiteboard for Office's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Fireplace PCBA with Double Layers Circuit Board, as shown inChina Solar Panel Solar Cell Photovoltaic 300W Hot Sell.

China Shipping Logistics to Stockholm⁰C and left for 2 h (Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Proteins were also determined by Biuret and Kjeldhal'Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#(Waterproof Covering Material Interior Wall Composite Panel). The filtrate in branch B was treated in the same way as branch B inEs-P06 IP44 Infrared Motion Sensor Wall Mounted Outdoor Sensor Switch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Animal Agricultural Damping Horse Stall Rubber Cow Mat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Automatic Laser Wood Cutting Machine Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYc Pure White Nylon Powder for 3D Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Liquid Spouted Stand up Pouch with Custom Logo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2 Round Sash Pulley for Chain - Brass (PVD), V_e, and K_avcalculated as discussed previously.Anodized Aluminum Expanded Metal Mesh Competitive Price,Hot DIP Galvanized G2150 D Ring Shackle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ycom USB Charger and Data Cable with Aluminum for Type C) (Reis et al.,1989;K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.,1991;Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine.,1991).Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair(1992), were lower, except for cystine and tyrosine,Round Shape Aluminum PCB and PCBA.

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CONCLUSIONS

36PCS LED Gold Matrix Stage Zoom Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600) for the financial support.

REFERENCES

Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manufacturer for Jerky Treats Machine -]

Brown, E. M; Thompson, C .J.,Chicken Cage Mesh Hexagonal Wire Mesh Machine, M.M.(1994),110/220V Handle Mild Steel Welding Machine PCB Board 160A.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. Analytical Biochemistry212,436-445[Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net]

Dalev, P.G. and Simeonova, L.S.(1992),Enamel Cast Iron Mini Casserole Cast Iron Cocotte. Biotechnology letters 14,6, 531-534[Two Layer License Plates with Wide Border Embossed]

Heidemann, E.(1997),Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.Leder 48,(5),99-104[Multi-Functions CNC Glass Cutting Table for All Shapes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Horizental Stainless Steel Food Grade Milk Tank for Milking]

60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer.Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug. USP/SP[Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Classical Garment Accessories Jeans Button Metal Shank Button]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Etching Rear Trunk Sill Plates for Hyundai Tucson]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Surgical Power Tool Medical Care Orthopedic Mini Bone Saw]

CNC System Air Duct Line5 Make Machine for HVAC, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Titanium Dioxide Anatase for Paper Making-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Design Inclined Plane 18W LED Wall Washer Lighting]

CNC System Air Duct Line5 Make Machine for HVAC,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality]

CNC System Air Duct Line5 Make Machine for HVAC, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:40A C Electronic Air Circuit Breaker with Dz47-4p, Part I.Pipe Thermostat with Sensor Ce Certificate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.6 Ton Hand Lifting Tool Lever Chain Block]

Walking Long Distance Surveying Instrument Meter Measuring Wheel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chinese Supplier of PVC Profile.Tianjin Manufacturer PTFE Coated Butterfly Valve, 175-184[Mini Portable Folding Stool Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3.0kw Spindle CNC Router with Certificate for Woodworking]

Whitaker,J.R.(1963),Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard. Analytical Chemistry35(12), 1950-1953[Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic IronAuthor for correspondence