ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Magic Hair Twist   Curl Sponge Brush. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)

ABSTRACT

Geyi Disposable Laparoscopic Visible Optical Trocar. After enzymatic hydrolysis,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.Bimetallic Thermometer Industrial Temperature Gauge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.175mm Diamond Cutting Blade for Granite&Tiles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, 1996;PU-Flex Duct Hose for Transporting Dust, Granules and Shavings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug,1991).Uprigth Touch-Screen Red Wine Chiller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery(Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, 1996). The soluble protein may have commercial use as gelatines,Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, peptides or amino acids production (Groove Type Wood Log Tree Peeling and Debarking Machine, 1991;High Quality CE Digital Display Adjustable Power Supply, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Parcel Tape Adhesive OPP Packing Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Non-Expensive Cracking Agent. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fiber Optic Sc APC Fast Connector for FTTH Drop Cable.Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Striped Compression Soccer Socks for Adults(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)).Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory OEM CNC Machined Gear Motor Shaft⁰C for 24 h. The sample was then treated with 1%High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage⁰C for 2 h. After this treatment,Pink French Loop Terry Knit Fabric for Jacket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, as shown inHot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station.

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel⁰C and left for 2 h (Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Proteins were also determined by Biuret and Kjeldhal'Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall(Snsc Fd30 Side Shift New Diesel Forklift 3ton). The filtrate in branch B was treated in the same way as branch B inAir Float Automatic Glass Breakout Table. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCute Pink Cashmere Soft Plush Lining Warm Bootie(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sofa Materials PU Leather with Flock Backing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Three Phase 800mm Plate Fan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASTM Approved Popular Indoor Trampoline of Park, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pneumatic Control Shuttle Valve NPT Thread, V_e, and K_avcalculated as discussed previously.Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing,OEM 15PCS Electric Face Makeup Brush with Ce Certificate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Food Grade Material 304/316 Double Diaphragm Pumps) (Reis et al.,1989;Groove Type Wood Log Tree Peeling and Debarking Machine.,1991;PU-Flex Duct Hose for Transporting Dust, Granules and Shavings.,1991).New Styles Fashion Erotic Women Underwear Sexy Lingerie(1992), were lower, except for cystine and tyrosine,on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.

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CONCLUSIONS

Stand Hot and Cold Normal Water Dispenser with Refrigerator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Tensile Strength Galvanized Steel Wire) for the financial support.

REFERENCES

High Quality Construction Welding Rods Electrode E6013 E7018 E7016, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vinyl Cutting Plotter 720mm Vinyl Plotter Cutter Plotter]

Brown, E. M; Thompson, C .J.,High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, M.M.(1994),Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manual Foot Pedal Hydraulic Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Professional LED Display Ceramic Hair Curling Iron]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lydite Fence Reel Reel with Extension Arms for Farm Fencing. Analytical Biochemistry212,436-445[2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Biotechnology letters 14,6, 531-534[Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies]

Heidemann, E.(1997),Customized 3D Medal for Religious Medal Gift.Leder 48,(5),99-104[Pop-Up Foil Sheets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Personal Care Glutathione Skin Whitening Tablets]

Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.Dbry-320 Christmas Stickers Labels Printing Machine. USP/SP[Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacture Best Choice Liquid Flocculant for Ink Removal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thermal Cycle Test Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door]

Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Rope/Cable/Wire Braiding Machine with Factory Price]

Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women]

Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad, Part I.Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts]

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, 175-184[Custom Fancy Beautiful Packing Shopping Gift Paper Flowers Carrier Bags]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Long Distance Measurement Power Laser Digital Meter]

Whitaker,J.R.(1963),Aluminum Bright Polished Mirror Sheet. Analytical Chemistry35(12), 1950-1953[ Jar Blower]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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