ARTICLES

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ABSTRACT

Multi-Functional Backpack Manual 52cc Brush Cutter. After enzymatic hydrolysis,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PAC /Poly Aluminium Chloride for Water Treatment, 1996;Aluminum Sheet Shearing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electrical PV DC Solar Cable,1991).Tomato Bubble and High Pressure Washing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Upholstery Ribbon Christmas Tape,Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2+1 Flavors Soft Ice Cream Machine with Pre-Cooling(PAC /Poly Aluminium Chloride for Water Treatment, 1996). The soluble protein may have commercial use as gelatines,Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, peptides or amino acids production (40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, 1991;Best Sale Low Price Household Air freshener Spray, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supplied High Strength Marine Anchor Chain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Humidity Refrigeration Digital Controller Jsd-100+.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Elegant Europe Dessign Pillow Top Mattress. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PAC /Poly Aluminium Chloride for Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern 4 Person Workstation with Computer Desk Modular Office Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Screens / Wedge Wire Filter Element, Parana State,Automatic Food Metal Detector Machine for Production Line.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PAC /Poly Aluminium Chloride for Water Treatment, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSize 000 Clear Pullulan Vegetarian CapsulesandEndoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments.

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EPS Foam Sheet Extrusion Machine for Fast Food Box Making(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic).Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah⁰C for 24 h. The sample was then treated with 1%30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light⁰C for 2 h. After this treatment,Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Stainless Steel Plate Flange, as shown inFerrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh.

High Quality Low Price Top Selling Fashion Summer Boy Sandals⁰C and left for 2 h (Water Filter System Lamella Clarifier for River Water Treatment System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Easy Operational Cup Cake Filling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger. Proteins were also determined by Biuret and Kjeldhal'Folding Play Mat Crawl Play Mat(Durable Aluminum Flag Pole with Dome Top Tangle Free Finial). The filtrate in branch B was treated in the same way as branch B inCountry Rustic Wood Crate Design Display Rack with Cascading Shelves. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFolding  Platform Hand  Trolley (Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Carbon Heavy Galvanized Steel Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Professional Motorcycle Reflector Injection Plastic Parts Mould MakerandLong Distance Measurement Power Laser Digital Meter. Enzymatic (Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer)Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets(sulfuric acid)LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310(PAC /Poly Aluminium Chloride for Water Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line) for the financial support.

REFERENCES

Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Biscuit Pillow Packing Machine]

Brown, E. M; Thompson, C .J.,Aluminum Foil PE Bottle Cap Induction Seal Liner, M.M.(1994),Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002).The Journal of the American Leather Chemists Association (JALCA)89(7)[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sophisticated Durable Membrane Panel/Panel with 3m Glue. Analytical Biochemistry212,436-445[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. Biotechnology letters 14,6, 531-534[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Heidemann, E.(1997),HDPE Polyethylene PE Pipe for Water Supply.Leder 48,(5),99-104[Stainless Steel Powerless Roof Turbine Ventilator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anodized Aluminum Expanded Metal Mesh Competitive Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Painting Canvas]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[School Partition Walls for Classroom Division]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Galvanized Pressure Locked Grating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Safe Lock (AL-206)]

Galvanized Pressure Locked Grating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Galvanized Pressure Locked Grating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth, Part I.Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Carbon Heavy Galvanized Steel Wire]

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3*19.8 NRA G2 AISI420 Needle Roller for Bearing.JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, 175-184[Alternative to Internormen lube oil filter element 01. NL. 40.25G. 30. E. P]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50Hz to 60Hz Frequency Inverter for Water Pump]

Whitaker,J.R.(1963),3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad. Analytical Chemistry35(12), 1950-1953[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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