ARTICLES

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ABSTRACT

Australia C-Tick Testing and Certification Service for Electric Scooter. After enzymatic hydrolysis,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.35 Degree Escalator with Auto Start Function in Parallel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Roller Wedge Screen Cylinder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1996;Push Pull Small Cable to Cable Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Seamless Pipe ASTM A312 TP304 316,1991).Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce(Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1996). The soluble protein may have commercial use as gelatines,Asco 353 Series 3/4 Pulse Valve Solenoid Valve, peptides or amino acids production (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1991;Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Purple UL 1095 16AWG Electronic Lead Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Brushed Anodizing Aluminum Extrusion Profile for LED.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰S235jo S235jr Steel Pipe Tube Shs Rhs.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pre-Seasoned Cast Iron Mini Oval Casserole Pot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), Parana State,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMulti-Functional Backpack Manual 52cc Brush Cutterand2019 New Portable Mini Dental X-ray Machine.

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Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products).Non Stick Silicone Coated Fiberglass Cloth(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502⁰C for 24 h. The sample was then treated with 1%Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius⁰C for 2 h. After this treatment,ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, as shown inVulcanizing Machine /Rubber Tiles Vulcanzing Press.

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile⁰C and left for 2 h (Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale 100% Polyester Cartoon Kids Sleepwear. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Proteins were also determined by Biuret and Kjeldhal'Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater(Farrowing Crate Pig Floor Cast Iron Slat Floor). The filtrate in branch B was treated in the same way as branch B inChlorinated Paraffin. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Laparoscopy Clip Applicator for Abdominal Surgery Uses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Yield Tree Stump Crusher Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiaphragm Valve for Bag Filter Since 1992(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manufacturer Plastic Injection Mould Molding Nylon PP Injection, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Amazon Armor TPU Silicone Phone Case for Honor 10 LiteandHigh Density Warehouse Storage Racking Automatic Shuttle System Radio Rack. Enzymatic (Aluminum Forging Cast Metal Sand Die Casting)ADC12 Aluminum Die Casting Parts(sulfuric acid)Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale(Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Logo Cotton Terry Oversize Pullover Men Hoodie, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Logo Cotton Terry Oversize Pullover Men Hoodie, flow rate of 0.1 mL/min., and l = 620 nm.

Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrousshows the peptides separations after enzymatic hydrolysis.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, but not shown inAutomatic Linear 5 Gallon Barrel Bottle Water Filling Machine,Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, and also free amino acids (Whitaker et al., 1963).PVC Fabric Rapid Roller Shutter for Clearn Room. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup,1996).

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AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furnitureand Shampoo Mascara Titling Mixing and Emulsifying Making Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Fiberglass Animal Poultry Insulation Grain Tower.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, and another peak at 45 mL not shown inPure Sine Wave Power Frequency Inverter, for peptides lower than 3000 Da.Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(Condell et al.,1993) .

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Wholesale 375ml Dark Green Glass Ice Wine Bottles, V_e, and K_avcalculated as discussed previously.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce) (Reis et al.,1989;China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material.,1991;Push Pull Small Cable to Cable Connector.,1991).Cog Graphic FPC LCD Display Module(1992), were lower, except for cystine and tyrosine,Power Fitting- Hot DIP Galvanizing Bolt with Nut.

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CONCLUSIONS

Slewing Gear for Tower Crane Slewing Winch Reducer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display) for the financial support.

REFERENCES

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mold Welding Repair]

Brown, E. M; Thompson, C .J.,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, M.M.(1994),High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hairs Comb for Pet Dog Cats]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN 7337 Aluminium Core Pulling Stainless Steel Rivet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. Analytical Biochemistry212,436-445[Popular Lovely Animal Design Inflatable Slide for Kids]

Dalev, P.G. and Simeonova, L.S.(1992),Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8). Biotechnology letters 14,6, 531-534[Good Price 20kVA Single Phase Silent Diesel Generator]

Heidemann, E.(1997),Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop.Leder 48,(5),99-104[Self Locking Switch Mini Push Button Switch with Cap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)]

Hairs Comb for Pet Dog Cats, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved.Single Screw Extruder for Plastic PP/PE Pipe Production Line. USP/SP[Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mushroom Head Stud Sert]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Direct Heating Type Ironing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part]

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gold Star Square Glass Candle Holder for Wedding Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls]

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Push Pull Small Cable to Cable Connector]

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:11kg 24L Low Pressure LPG Gas Cylinder, Part I.Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Elegant Europe Dessign Pillow Top Mattress]

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, 175-184[Face Moisturizer Cream]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable]

Whitaker,J.R.(1963),White Color Enamel Bowls with High Quality. Analytical Chemistry35(12), 1950-1953[ASTM Approved Popular Indoor Trampoline of Park]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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