ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Women Fashion Winter V Neck Long Sleeve Clothes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line

ABSTRACT

3D CNC Router for Wood Cutting Machine. After enzymatic hydrolysis,Stainless Steel Screens / Wedge Wire Filter Element.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EPS Foam Sheet Extrusion Machine for Fast Food Box Making;Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, 1996;Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine,1991).High Quality Stainless Steel Plate Flange. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Low Price Top Selling Fashion Summer Boy Sandals,Easy Operational Cup Cake Filling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger(30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, 1996). The soluble protein may have commercial use as gelatines,Folding Play Mat Crawl Play Mat, peptides or amino acids production (Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, 1991;Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Folding  Platform Hand  Trolley ., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pump Flower Sprinkler with Automatic Watering System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field.Low Carbon Heavy Galvanized Steel Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Long Distance Measurement Power Laser Digital Meter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Dipped Galvanized Oval Wire Brazil⁰C and left for 2 h (High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film. Proteins were also determined by Biuret and Kjeldhal'FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit(Wooden Grain Perforated Panel for Metal Mesh Curtain Wall). The filtrate in branch B was treated in the same way as branch B in12mm 15mm Bronze Fire Resistant Premium Tempered Glass with Packaging. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Foil PE Bottle Cap Induction Seal Liner. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HDPE Polyethylene PE Pipe for Water Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket. 316L)shows the peptides separations after enzymatic hydrolysis.5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCatering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, but not shown in22 High Rubber Mixing Mill,3*19.8 NRA G2 AISI420 Needle Roller for Bearing, and also free amino acids (Whitaker et al., 1963).JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light,1996).

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Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, V_e, and K_avcalculated as discussed previously.Portable Mini Fridge with Light Box,Agriculture Film for Corn Silage Wrap Baler. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electrical PV DC Solar Cable) (Reis et al.,1989;Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.,1991;Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel.,1991).Cheap Gabion Wire Mesh Box(1992), were lower, except for cystine and tyrosine,Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film.

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CONCLUSIONS

Stainless Steel Double Layer Filter USD in POY Machines. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310) for the financial support.

REFERENCES

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM USB Electric Adult Sex Toy Double Vibrator]

Brown, E. M; Thompson, C .J.,Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, M.M.(1994),Graphite Ring Good Lubrication.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[175mm Diamond Cutting Blade for Granite&Tiles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Tower 6-Axis Screwdriver Machine Robot Manufacturer. Analytical Biochemistry212,436-445[Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Mslqs-P Needle-Free Injector/Needleless Syringe Price. Biotechnology letters 14,6, 531-534[Sinicline Custom Brand with Plastic Pouch Hangtag for Hats]

Heidemann, E.(1997),YAMAHA Yv100II Nozzle Horder Square Head Short Type.Leder 48,(5),99-104[K102z059-130]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Forming Pushing Elbow Making Machine]

High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Juice Doypack Packing Machine (ZLDB-4).Stainless Steel Wok with Soft Touch Handle. USP/SP[Greenhouse Fixing Film Profile Lock Channel and Spring Wire]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[20FT New Shipping Container 40FT Dry Cargo Container]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8mm Outdoor LED Screen for Video and Advertising]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack]

Funny Newest Design for Children Theme Outdoor Playground Park, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price]

Funny Newest Design for Children Theme Outdoor Playground Park,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2ton R Type Electric Wire Rope Hoist with Single Speed]

Funny Newest Design for Children Theme Outdoor Playground Park, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG, Part I.Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Peb Steel Structure Building Materials H-Section I Beam Steel]

Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Strength GRP Cooling Towers for Mechanical Cooling.Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash, 175-184[Cheap Wood Screws High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Whitaker,J.R.(1963),Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief. Analytical Chemistry35(12), 1950-1953[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Must 4kw 2HP Pump Power Inverter Ags Bts AvailableAuthor for correspondence