ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine

ABSTRACT

Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use. After enzymatic hydrolysis,Manual Foot Pedal Hydraulic Pump.860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Precision Infusion Set with Filter and Needle for Single Use, 1996;Low Price Sweep Frequency Response Analyzer for Power Transformer Test., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500,1991).Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT,Mining Machinery Sand Vibrating Sieve for River Sand,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve(Disposable Precision Infusion Set with Filter and Needle for Single Use, 1996). The soluble protein may have commercial use as gelatines,PRO Audio Loudspeaker PA Speaker System Line Array Speaker, peptides or amino acids production (Palet Coil Nail Welding Machine/Nail Making Machine, 1991;Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality MPO Singlemode Duplex Fiber Optic Adapter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hotel Bedding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Supplier V Wedge Wire Johnson Water Well Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Precision Infusion Set with Filter and Needle for Single Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles.10A MPPT Solar Controllers PV 60V for off-Grid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Outlet Automatic Grain Tower Dryer Drying Equipment, Parana State,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine.Mesh Bag Packing 20kg Pure White Garlic, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Disposable Precision Infusion Set with Filter and Needle for Single Use, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePneumatic Control Shuttle Valve NPT ThreadandNew Fashion PVC Sequins Adjustable Women Brace Suspenders.

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Supreme 524 Mv Sea Kayak(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture).Trunk Organizer Foldable Water Proof(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)⁰C for 24 h. The sample was then treated with 1%Diesel Generator Parts AVR Ki-Davr-95s for Kipor⁰C for 2 h. After this treatment,Gld Brand Padlock Brass Popular in 5 Continents  . The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)No Lead Bronze Flange Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, as shown in Eye Steam Mask.

Snsc Fd30 Side Shift New Diesel Forklift 3ton⁰C and left for 2 h (Carrier Roller for Sany Excavator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. Proteins were also determined by Biuret and Kjeldhal'Laptop Bag College Men Backpack Leather Back Pack Bags(Hot DIP Galvanized G2150 D Ring Shackle). The filtrate in branch B was treated in the same way as branch B inBag leather. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pet Dog Grooming Rake Dematting Tool Shedding Comb.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFarm Tools and Equipment 7ton Backhoe Loader Construction Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Food Metal Detector Machine for Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi-Functional Backpack Manual 52cc Brush Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Oxide Non Woven Polishing Wheel for MachineandModern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Enzymatic (Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine)Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm(sulfuric acid)White Cotton Satin Hotel Duvet Cover (DPFB8055)(Disposable Precision Infusion Set with Filter and Needle for Single Use, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, flow rate of 0.1 mL/min., and l = 620 nm.

PAC /Poly Aluminium Chloride for Water Treatment.Concrete Floor Grinding Disc Machine for Four Segmentsshows the peptides separations after enzymatic hydrolysis.Aluminum Sheet Shearing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, but not shown inPlanter Moving Round Planter Saucer Casters Plant Trolley (M),Electrical PV DC Solar Cable, and also free amino acids (Whitaker et al., 1963).Tomato Bubble and High Pressure Washing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Disposable Precision Infusion Set with Filter and Needle for Single Use,1996).

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Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, V_e, and K_avcalculated as discussed previously.40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse,Best Sale Low Price Household Air freshener Spray. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Slip Resistant Corrugated Protection Entrance Rubber Floor Mat) (Reis et al.,1989;Palet Coil Nail Welding Machine/Nail Making Machine.,1991;Low Price Sweep Frequency Response Analyzer for Power Transformer Test.,1991).Factory Supplied High Strength Marine Anchor Chain(1992), were lower, except for cystine and tyrosine,Humidity Refrigeration Digital Controller Jsd-100+.

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CONCLUSIONS

Elegant Europe Dessign Pillow Top Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price) for the financial support.

REFERENCES

8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nano Bubble Generator with Good Gas-Liquid Dissolving Effect]

Brown, E. M; Thompson, C .J.,Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, M.M.(1994),Modern 4 Person Workstation with Computer Desk Modular Office Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Separating Gold Brass Zippers for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. Analytical Biochemistry212,436-445[Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Women Fashion Winter V Neck Long Sleeve Clothes. Biotechnology letters 14,6, 531-534[Dia. 5.5mm Bonding ND: YAG Laser Rod Lens]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Amazon Armor TPU Silicone Phone Case for Honor 10 Lite: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.sdjtec.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

EPS Foam Sheet Extrusion Machine for Fast Food Box Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

EPS Foam Sheet Extrusion Machine for Fast Food Box Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flat Pack Temperary Container House 20FT 40FT]

EPS Foam Sheet Extrusion Machine for Fast Food Box Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, Part I.30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Engieered Veneer Faced Commericial Plywood]

Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine.High Quality Stainless Steel Plate Flange, 175-184[3 Phasen Ac Motor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

Whitaker,J.R.(1963),High Quality Low Price Top Selling Fashion Summer Boy Sandals. Analytical Chemistry35(12), 1950-1953[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27Author for correspondence