ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Toilet Roll Tissue Wrapping Machine with Auto - Counting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine

ABSTRACT

Orange Monofilament Fishing Tackle Nylon Fish Net. After enzymatic hydrolysis,Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002).By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Diameter 1300mm Lifting Electro Magnet for Cast Iron;High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Evaporator Finned Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, 1996;Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100%Polyester Spandex Hotel Chair Cover (DPH7022),1991).Factory Supply Perforated Metal Mesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Turning Center, CNC Horizontal Turning Milling Machine,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use(Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, 1996). The soluble protein may have commercial use as gelatines,Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, peptides or amino acids production (Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, 1991;Factory Wholesale Zinc Plated Carbon Steel Hex Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Cheese Making Machine Electric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.100W Dimmable LED Corn Bulb with Meanwell Driver-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet and Animal Food Extrusion Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)).Biomass Drum Wood Chipper with Competitive Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags⁰C for 24 h. The sample was then treated with 1%Forged Steel Pressure Sealed Bonnet Globe Valve⁰C for 2 h. After this treatment,Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, as shown inHigh Quality Construction Welding Rods Electrode E6013 E7018 E7016.

Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure⁰C and left for 2 h (10ml Round Clear Nail Polish Bottle with Brush Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.80W All in One Outdoor LED Street Light Solar Lamp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Safety Vinyl PVC Flooring. Proteins were also determined by Biuret and Kjeldhal'Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation(12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad). The filtrate in branch B was treated in the same way as branch B inDemountable Wheel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Horizental Stainless Steel Food Grade Milk Tank for Milking.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi Parameter Small Digital Domestic Water Meter TDS Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Picosecond Laser Tattoo Removal Skin Care Medical Instrument⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ideabond Stone Texture ACP Aluminum Composite Panel for OutdoorandHospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart. Enzymatic (SUS316 Hexagon Socket Set Screws with Cone Point, DIN914)Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(sulfuric acid)Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor(Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, flow rate of 0.1 mL/min., and l = 620 nm.

Explosion-Proof Housing Sealing Dispensing Machine.CNC Machining Turning Anodized Aluminum Worm Gearshows the peptides separations after enzymatic hydrolysis.Tube Mills for Thin Wall Tubing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, but not shown inGraphic 128X128 Dots Cog LCD,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core, and also free amino acids (Whitaker et al., 1963).Car Badge Custom Emblem Auto Logo Sign for Advertising. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear,1996).

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CONCLUSIONS

Silver 8 Inch Double Thick Round Cake Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (V-32 New Special UPS Solar Battery Cabinet Box Case) for the financial support.

REFERENCES

High Precision Machining Auto Parts Metal Contact, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.czmtev.com]

Brown, E. M; Thompson, C .J.,Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, M.M.(1994),Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sophisticated Durable Membrane Panel/Panel with 3m Glue]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Car Mattress Back Inflatable Air Bed Car]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Paper Sticky Lint Remover Roller/Pet Hair Roller. Analytical Biochemistry212,436-445[Decorative Aluminum Solid Panel for Commercial Building]

Dalev, P.G. and Simeonova, L.S.(1992),Safe Durable Scaffolding Trapdoor Plank for Construction. Biotechnology letters 14,6, 531-534[Medical Human Body Thermometer]

Heidemann, E.(1997),China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.Leder 48,(5),99-104[Factory Supplier Ozone Anion Oil Diffuser Air Purifier Air Cleaner]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[35 Degree Escalator with Auto Start Function in Parallel]

Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Various Sublimation Blank Home Supply Fabric Bath Towel etc.High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. USP/SP[CH Series Pid Intelligent Temperature Controller CH402]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Metal Button Crystal Shank Button for Garment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High-End Business Magnet Leather Ring Binder Notebook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cracker Multi Rows Trayless Packaging Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine]

Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Quality Bending Metal Mould with All Kinds Metal Materials-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lower Noise Colled Rolled Ball Screw]

Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized Eye Grab Bend Hook]

Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, Part I.Milling Precision Stainless Steel CNC Machinery Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chinese Galvanized Rectangular Tube for Buildings Materials]

U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, 175-184[www.sunllent-solar.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UF Membrane Aqu160 with Competitive Price and High Quality]

Whitaker,J.R.(1963),Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10. Analytical Chemistry35(12), 1950-1953[PVC Connector Sleeve Soft Plastic Battery Terminal Covers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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