ARTICLES

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ABSTRACT

China Customized Chilled Cast Iron Roll. After enzymatic hydrolysis,IMTEK High Precision and Rigidity Linear Guides for CNC machinery.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Shipping Logistics to Stockholm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1996;Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#,1991).Animal Agricultural Damping Horse Stall Rubber Cow Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Automatic Laser Wood Cutting Machine Price,Yc Pure White Nylon Powder for 3D Printing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats(Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1996). The soluble protein may have commercial use as gelatines,Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, peptides or amino acids production (High Quality Liquid Spouted Stand up Pouch with Custom Logo, 1991;Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Modern Pharmacy Vacuum Filter Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Cotton Luxury Printed Bedding Comforter Set. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mouse Pad with Gel Wrist Rest.Custom Size Christmas Gift Personalized Watch Boxes for Men-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Selling Quality Aluminum Acetylene Gas Cylinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, Parana State,Chinese Galvanized Rectangular Tube for Buildings Materials.Factory Direct Sale Single Cloth Hanger Hook, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men BabyandNew Design Inflatable Water Playground Amusement Park with Water Slide Pool.

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2 Round Sash Pulley for Chain - Brass (PVD)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Car Badge Custom Emblem Auto Logo Sign for Advertising).Anodized Aluminum Expanded Metal Mesh Competitive Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot DIP Galvanized G2150 D Ring Shackle⁰C for 24 h. The sample was then treated with 1%Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair⁰C for 2 h. After this treatment,Round Shape Aluminum PCB and PCBA. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)36PCS LED Gold Matrix Stage Zoom Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, as shown in5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter.

Chicken Cage Mesh Hexagonal Wire Mesh Machine⁰C and left for 2 h (Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.110/220V Handle Mild Steel Welding Machine PCB Board 160A. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. Proteins were also determined by Biuret and Kjeldhal'Enamel Cast Iron Mini Casserole Cast Iron Cocotte(China Tower 6-Axis Screwdriver Machine Robot Manufacturer). The filtrate in branch B was treated in the same way as branch B inBarb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC System Air Duct Line5 Make Machine for HVAC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Titanium Dioxide Anatase for Paper Making, pH 3.25., mobile phase B: buffer solution of 0.01 mol/40A C Electronic Air Circuit Breaker with Dz47-4p, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Food Grade Material 304/316 Double Diaphragm PumpsandPipe Thermostat with Sensor Ce Certificate. Enzymatic (Walking Long Distance Surveying Instrument Meter Measuring Wheel)Chinese Supplier of PVC Profile(sulfuric acid)Tianjin Manufacturer PTFE Coated Butterfly Valve(Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Price of Automatic Sludge Dewatering Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade Transparent Cookie Airtight Plastic Container Jars) for the financial support.

REFERENCES

Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Food Trailers]

Brown, E. M; Thompson, C .J.,Worm Gear Operation Wafer Lined F3 Butterfly Valve, M.M.(1994),Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Rope/Cable/Wire Braiding Machine with Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. Analytical Biochemistry212,436-445[Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts]

Dalev, P.G. and Simeonova, L.S.(1992),Positive Drive Turn Belt for Spiral Freezing Equipment. Biotechnology letters 14,6, 531-534[Long Distance Measurement Power Laser Digital Meter]

Heidemann, E.(1997),Chinese Herb Medicine Angelica Root Extract.Leder 48,(5),99-104[2 Layers Metal Wire Kitchen Dish Rack Plastic Board No. Dr16-Bp03]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Jar Blower]

Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),S Series China Generator Helical-Worm Crushers Reduction Box.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale. USP/SP[110/220V Handle Mild Steel Welding Machine PCB Board 160A]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hardware CNC Machinery Milling Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Original Testo 174t Mini Temperature Data Logger No. 0572 1560: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Body Jewelry Sticker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT]

Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Laundry Used Gas Water Heater with CE/Asme Certificate]

Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle]

Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, Part I.Whole Set Gas Spring Strut for Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2.0mm Yellow Markers for Fiber Optic Multi Patch Cord]

Dia. 5.5mm Bonding ND: YAG Laser Rod Lens, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining Turning Anodized Aluminum Worm Gear.China Solar Panel Solar Cell Photovoltaic 300W Hot Sell, 175-184[Automated Tunnel Segment Carousel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stand Hot and Cold Normal Water Dispenser with Refrigerator]

Whitaker,J.R.(1963),Vacuum Coating Machine Water Chiller (CW-6300AN). Analytical Chemistry35(12), 1950-1953[ACP Aluminium Cmposite Panel Cladding Extrusions]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hotel BeddingAuthor for correspondence