ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hotel SPA Swimming Pool, Sport Room, Bathroom Towel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Powerful Cleaning Colorful Scouring Pad for Kitchen Use

ABSTRACT

500t Plywood Hot Press Woodworking Machine (12-25 layers). After enzymatic hydrolysis,10ml Round Clear Nail Polish Bottle with Brush Cap.Full Automatic 4000b/h 500ml Plastic Jar Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine;5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hermetic Sliding Lead Door, X Ray Room Door, 1996;Laundry Used Gas Water Heater with CE/Asme Certificate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp,1991).2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Accept OEM New Products Team Various Colors Gold Ribbon,Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh(Hermetic Sliding Lead Door, X Ray Room Door, 1996). The soluble protein may have commercial use as gelatines,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, peptides or amino acids production (Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair, 1991;What′s Kind of Machine for Making Organic Fertilizer Granules, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Home and Hospital Electric Patient Lift.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hermetic Sliding Lead Door, X Ray Room Door, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality.Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DC 12V Power Relay for Smart House(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter⁰C and left for 2 h (High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap. Proteins were also determined by Biuret and Kjeldhal'Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening(Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck). The filtrate in branch B was treated in the same way as branch B inProfessional Customized Automatic Spray Paint Production Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.) for the financial support.

REFERENCES

Fashion Metal Button Crystal Shank Button for Garment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[108kw Cooling System Water Chiller with Bitzer Screw Comprossor]

Brown, E. M; Thompson, C .J.,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), M.M.(1994),10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. Analytical Biochemistry212,436-445[Accept OEM New Products Team Various Colors Gold Ribbon]

Dalev, P.G. and Simeonova, L.S.(1992),ABC-508 Brake System Cleaning and Fluid Exchanging Machine. Biotechnology letters 14,6, 531-534[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Industrial Grade Anhydrous Sodium Sulfate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[S Series China Generator Helical-Worm Crushers Reduction Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tin Lead Solder Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pre-Seasoned Cast Iron Baking Stone Cookware]

Professional Factory Supply Design Cat Tree Pet Product, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Professional Factory Supply Design Cat Tree Pet Product,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Concrete Spare Part Electrical System Central Distribution Box]

Professional Factory Supply Design Cat Tree Pet Product, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Peb Steel Structure Building Materials H-Section I Beam Steel, Part I.Aluminum Oxide Non Woven Polishing Wheel for Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[No. 1062 20PCS Tools]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

Whitaker,J.R.(1963),Famous Brand Luxury Packaging Box Gift Box. Analytical Chemistry35(12), 1950-1953[Wholesale Genuine Leather Credit Card Mens Wallet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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