ARTICLES

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^IICNPq/RHAE/biotechnology-Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail

ABSTRACT

High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. After enzymatic hydrolysis,Stainless Steel Clarifier Tank for Water Treatment.Carrier Roller for Sany Excavator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes;Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, 1996;Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle,1991).High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce,Dry Air Generator GF Series,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture(Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, 1996). The soluble protein may have commercial use as gelatines,Advanced Unlocking Tool for Locksmith, peptides or amino acids production (Screen Printing Materials and Equipments Screen Printing Frames, 1991;China Supplier Hard Ware Fittings Gas Spring for Tool Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855.CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Horizontal Refrigeration

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (43 Glass Beads Cat Eye Aluminum Road Stud).High Speed Copper Wire Braiding Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Safe Durable Construction Scaffolding Plank for Building⁰C for 24 h. The sample was then treated with 1%Different Size of Ring NdFeB Magnet⁰C for 2 h. After this treatment,Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Welding Structure's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carboxymethyl Cellulose CAS No. 9004-32-4, as shown inWater Cooled Low Temperature Screw Chiller for Industrial Workshop.

Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher⁰C and left for 2 h (China Prefab Steel Building for Garment Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,150kg Per Hour Frozen French Fries Production Line. Proteins were also determined by Biuret and Kjeldhal'Automatic Professional LED Display Ceramic Hair Curling Iron(Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag). The filtrate in branch B was treated in the same way as branch B inchinapucornice. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVulcanizing Machine /Rubber Tiles Vulcanzing Press(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Omni Newest Manager Desk Office Smart Office Table Office Staff Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Glass Lifting Machine with Sucker for Glass Movementsand30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. Enzymatic (Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage)Full-Auto CNC Cutting Machine for Architecture Glass Sheet(sulfuric acid)Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product(Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Forming Pushing Elbow Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Forming Pushing Elbow Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.Flat Car Sun Roof Tent Awningshows the peptides separations after enzymatic hydrolysis.Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAtf-805 Auto Car Transmission System Flushing Machine, but not shown inAutomatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine,As568 Standard Brown FKM Rubber O Ring for Sealing, and also free amino acids (Whitaker et al., 1963).The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets,1996).

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CONCLUSIONS

High Borosilicate Glass Baking Dish Set Clear. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce) for the financial support.

REFERENCES

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Petroleum Coke Recarburizer]

Brown, E. M; Thompson, C .J.,Embossing Roller for Tissue Paper, M.M.(1994),Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel.The Journal of the American Leather Chemists Association (JALCA)89(7)[6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates. Analytical Biochemistry212,436-445[Dn32 (11/4) BSPT Thread Air Actuated Piston Valves]

Dalev, P.G. and Simeonova, L.S.(1992),Colorful  Reflective Sticker Reflective Tape. Biotechnology letters 14,6, 531-534[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

Heidemann, E.(1997),Concrete Spare Part Electrical System Central Distribution Box.Leder 48,(5),99-104[Kids Lunch Box Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chicken Cage Mesh Hexagonal Wire Mesh Machine]

Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress.50Hz to 60Hz Frequency Inverter for Water Pump. USP/SP[Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roller Wedge Screen Cylinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Freshwater Pearl Butterfly Flower Bride Hairpin]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat Exchanger Bundle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women]

High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Elbow Forming Machine Gorelocker HVAC Duct Making Machine]

High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver]

High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator, Part I.PVC Cabinet Construction Board, White 18mm PVC Foam Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customize Milky Extrusion PC Cover]

2019 New Portable Mini Dental X-ray Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ss Reduced Port 2000wog 1PC Screwed Ball Valve.China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous, 175-184[6061 7075 2024 Aluminum Machining]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Whitaker,J.R.(1963),China Prefab Steel Building for Garment Factory. Analytical Chemistry35(12), 1950-1953[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion ProfilesAuthor for correspondence