ARTICLES

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ABSTRACT

Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0. After enzymatic hydrolysis,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes.Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pre-Seasoned Cast Iron Baking Stone Cookware;Compression Polyester Light Ankle Support Brace for Youth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality White PVC Rigid Foam Board for Iran Market, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, 1996;Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Precision Piston Ring Good Shape Ability High Quality Surface,1991).Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Farm Relief Plastic Automatic Air Release Valve,60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry(Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, 1996). The soluble protein may have commercial use as gelatines,Square Decorative Stainless Steel Crimped Wire Mesh, peptides or amino acids production (Mold Injection Molding Plastic Products White PC Housing, 1991;Sw Type BOPP Packaging Adhesive Tape Coating Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Metric Female Flat Seat Hose Fittings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi Color Spade Made of Steel and Panting.Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVaried Colored As568 Food Grade Soft Rubber Silicone O Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese Galvanized Rectangular Tube for Buildings Materials⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Customized Chilled Cast Iron Roll, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IMTEK High Precision and Rigidity Linear Guides for CNC machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mouse Pad with Gel Wrist Rest. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4 Way Stretch Running Gym Shorts Unique Women Short Pants) for the financial support.

REFERENCES

Custom Size Christmas Gift Personalized Watch Boxes for Men, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Excellent Quality Shell and Tube Heat Exchanger]

Brown, E. M; Thompson, C .J.,Best Selling Quality Aluminum Acetylene Gas Cylinder, M.M.(1994),Coal Mine Mini Electric Excavator with Quick Coupling.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ABC-508 Brake System Cleaning and Fluid Exchanging Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank. Analytical Biochemistry212,436-445[Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)]

Dalev, P.G. and Simeonova, L.S.(1992),Food Grade Transparent Cookie Airtight Plastic Container Jars. Biotechnology letters 14,6, 531-534[Slip Resistant Corrugated Protection Entrance Rubber Floor Mat]

Heidemann, E.(1997),Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.Leder 48,(5),99-104[Ceramic Roloc Sanding Disc for Aeroengine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Separating Gold Brass Zippers for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2 Round Sash Pulley for Chain - Brass (PVD): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Woman style Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts]

Anodized Aluminum Expanded Metal Mesh Competitive Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot DIP Galvanized G2150 D Ring Shackle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dia. 5.5mm Bonding ND: YAG Laser Rod Lens]

Anodized Aluminum Expanded Metal Mesh Competitive Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

Anodized Aluminum Expanded Metal Mesh Competitive Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, Part I.Round Shape Aluminum PCB and PCBA. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

36PCS LED Gold Matrix Stage Zoom Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China.Chicken Cage Mesh Hexagonal Wire Mesh Machine, 175-184[www.jb-hardware.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

Whitaker,J.R.(1963),110/220V Handle Mild Steel Welding Machine PCB Board 160A. Analytical Chemistry35(12), 1950-1953[Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)Author for correspondence