ARTICLES

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^IICNPq/RHAE/biotechnology-6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps

ABSTRACT

Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. After enzymatic hydrolysis,Full-Auto CNC Cutting Machine for Architecture Glass Sheet.Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Forming Pushing Elbow Making Machine;13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (As568 Standard Brown FKM Rubber O Ring for Sealing, 1996;The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card,1991).200W UFO LED High Bay Light Fixture for Interior Exterior Applications. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2),13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bathroom False Plastic Board Interior Decorative 3D Wall Panels(As568 Standard Brown FKM Rubber O Ring for Sealing, 1996). The soluble protein may have commercial use as gelatines,Mushroom Head Stud Sert, peptides or amino acids production (Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women, 1991;Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Borosilicate Glass Baking Dish Set Clear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossing Roller for Tissue Paper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (As568 Standard Brown FKM Rubber O Ring for Sealing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel.Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colorful  Reflective Sticker Reflective Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)).PVC Cabinet Construction Board, White 18mm PVC Foam Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 New Portable Mini Dental X-ray Machine⁰C for 24 h. The sample was then treated with 1%Ss Reduced Port 2000wog 1PC Screwed Ball Valve⁰C for 2 h. After this treatment,China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Prefab Steel Building for Garment Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, as shown in7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side.

Cable Tray with ′t′ Slots⁰C and left for 2 h (Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10 Gauge Galvanized Welded Wire Mesh for Construction Using. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Factory Inline Hockey Skate Puck. Proteins were also determined by Biuret and Kjeldhal'0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector(CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts). The filtrate in branch B was treated in the same way as branch B inAutomatic Truck Tire Changer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable PS Foam Fast Food Box Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Foska Good Quality Plastic Stapler Set with Staples⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting FittingsandPowerful Cleaning Colorful Scouring Pad for Kitchen Use. Enzymatic (500t Plywood Hot Press Woodworking Machine (12-25 layers))10ml Round Clear Nail Polish Bottle with Brush Cap(sulfuric acid)Full Automatic 4000b/h 500ml Plastic Jar Making Machine(As568 Standard Brown FKM Rubber O Ring for Sealing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50Hz to 60Hz Frequency Inverter for Water Pump) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, M.M.(1994),M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Titanium Dioxide Anatase for Paper Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts. Analytical Biochemistry212,436-445[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

Dalev, P.G. and Simeonova, L.S.(1992),DC 12V Power Relay for Smart House. Biotechnology letters 14,6, 531-534[10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light]

Heidemann, E.(1997),2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment.Leder 48,(5),99-104[Big Size Stainless Pipe/ Extra-Heavy Pipe/Thick Walled Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Greenhouse Fixing Film Profile Lock Channel and Spring Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Quality Bending Metal Mould with All Kinds Metal Materials]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019 Practical 6063 T5 Aluminium Extruded Wood Finish Profiles for Thermal Break Aluminum Windows]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF]

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, Part I.48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52.China Supplier Granular Coal Based Activated Carbon Filter Meida, 175-184[Travel Bottles Silicone Containers Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Shoulder Backpack Handbag School Bag]

Whitaker,J.R.(1963),Glass Cutting Table/CNC Glass Cutting Machine. Analytical Chemistry35(12), 1950-1953[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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