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ABSTRACT

FRP Water Tank GRP Fishing Tanks From Factory Supply. After enzymatic hydrolysis,Decorative House Stone Coated Metal Roof Tile.Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder;Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, 1996;Blue Steel Packing Strip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Powder and Wax Brush with Wooden Handle,1991).Cheap Price Paper Straws Individually Wrapped Drinking Straw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality,Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose(PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, 1996). The soluble protein may have commercial use as gelatines,Smelting and Heating Induction Machine (GW-HY187), peptides or amino acids production (Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem, 1991;Home Products 16′ Perforated Flex Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1220 Fork Length 3-Wheel Electric Forklift Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FM USB Radio Audio Power Amplifier for Home Bar Club. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable.Hot Sell Mechanical Bar Screen for Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glow Powder for Luminous Coating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

450ml Sublimation Stainless Steel Milk Bottle (Colorful), Parana State,Cold Rolled Stainless Steel Strip /Coils 410/430/409.Lower Noise Colled Rolled Ball Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminium / Stainless Channel Letter Auto Profile Bending Machine PriceandPVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle.

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Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels).3.0kw Spindle CNC Router with Certificate for Woodworking(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%800W Electric Motor Price Lawn Mower Motor Concrete Mixer⁰C for 24 h. The sample was then treated with 1%Air Cooled Split Type Ducted Air Conditioner AC Unit⁰C for 2 h. After this treatment,Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gym Fitness Equipment Adjustable Weight Bench, as shown in3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope.

Factory Sell P7.8 Transparent LED Display Screen⁰C and left for 2 h (Australia C-Tick Testing and Certification Service for Electric Scooter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Weichai Engine Truck Spare Parts Valve Cap 61500050105. Proteins were also determined by Biuret and Kjeldhal'Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber(Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints). The filtrate in branch B was treated in the same way as branch B in100uh inductor smd. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Charms for Jewelry Making (Sold Per Bag 10PCS Inside). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFused Quartz Powder 325mesh 500mesh 1250mesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing MachineandWaste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India. Enzymatic (Soft Baby Suites Dinosaure Pijamas Kigurumi)Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply(sulfuric acid)High Density Celuca Kitchen Cabinets PVC Foam Board(PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, flow rate of 0.1 mL/min., and l = 620 nm.

E171 Standrad Titanium Dioxide Food Grade A200.Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Blockshows the peptides separations after enzymatic hydrolysis.Elbow Forming Machine Gorelocker HVAC Duct Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLarge Slewing Rings Bearings for Wind Turbine Ring on Sale, but not shown in15W Aquarium Submersible UV Lamp Durable Germicidal Lamp,Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh, and also free amino acids (Whitaker et al., 1963).Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use,1996).

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CONCLUSIONS

ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Union Metal Steel Building and Construction Material) for the financial support.

REFERENCES

in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Loading Ambulance Stretcher]

Brown, E. M; Thompson, C .J.,Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500, M.M.(1994),W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carboxy Terminal Collagen Crosslinks Elisa Kits]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Popular Custom School Uniform Woven Badge Patch with Merrow Border]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cake/Cookies/Bread Automatic Package and Packing Machine. Analytical Biochemistry212,436-445[Pump Flower Sprinkler with Automatic Watering System]

Dalev, P.G. and Simeonova, L.S.(1992),Customize Milky Extrusion PC Cover. Biotechnology letters 14,6, 531-534[Rear Wheel Exchangeable Aluminum Manual Wheelchair]

Heidemann, E.(1997),European Good Quality Bathroom Simple Sliding Glass Shower Cabina.Leder 48,(5),99-104[Dental Intra Oral Scanner Runyes 3ds]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Body Infrared Burner Good Selling Gas Stove]

Flat Pack Temperary Container House 20FT 40FT, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Fruit Plum Cleaning Washing Machine with Brushes Spray. USP/SP[High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Socket Flange]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disposable Sterile Twist Top Blood Lancet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Quality Main Tee Grids for Gypsum Ceiling Tiles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup]

11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bamboo Tablet Stand Laptop Rack Bt-2212]

11.1V 2000mAh Lithium Polymer Battery Pack Customized Size,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift]

11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jh21 Series Pneumatic Power Press CNC Punching Machine, Part I.Coal Fried Steam Boiler for Industry with Double Drum. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting]

Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Filter Strainer.Granite Cutting Diamond Saw Blades Cutting Blade, 175-184[Pop up Metal/Stainless Steel Exhibition Fixture Retail Cabinet for Shop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Surprise! Range Hood Free! ! ! Juice Cart]

Whitaker,J.R.(1963),Steel Forged Automobile Spare Parts with Customized Drawing. Analytical Chemistry35(12), 1950-1953[Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game MachineAuthor for correspondence