ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. After enzymatic hydrolysis,Asco 353 Series 3/4 Pulse Valve Solenoid Valve.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle;Purple UL 1095 16AWG Electronic Lead Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Brushed Anodizing Aluminum Extrusion Profile for LED, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1996;S235jo S235jr Steel Pipe Tube Shs Rhs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing,1991).Pre-Seasoned Cast Iron Mini Oval Casserole Pot. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Square Wire Mesh Netting with SGS,Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display(PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1996). The soluble protein may have commercial use as gelatines,Outdoor Garden Save Energy with Solar Flame Wall Light, peptides or amino acids production (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), 1991;Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non Stick Silicone Coated Fiberglass Cloth.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcrAlcalase2.5 L DX,White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hot Sale 100% Polyester Cartoon Kids Sleepwear); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, Parana State,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.Laparoscopy Clip Applicator for Abdominal Surgery Uses, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging MachineandCustom Striped Compression Soccer Socks for Adults.

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aihdcam

High Yield Tree Stump Crusher Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA).Diaphragm Valve for Bag Filter Since 1992(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf⁰C for 24 h. The sample was then treated with 1%Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine⁰C for 2 h. After this treatment,Manufacturer Plastic Injection Mould Molding Nylon PP Injection. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, as shown inFactory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.

Aluminum Forging Cast Metal Sand Die Casting⁰C and left for 2 h ( Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ADC12 Aluminum Die Casting Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. Proteins were also determined by Biuret and Kjeldhal'Custom Logo Cotton Terry Oversize Pullover Men Hoodie(6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap). The filtrate in branch B was treated in the same way as branch B inRound Wireless Charging Ferrite. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSeikofire S-66 Palm OTDR Sm/mm Fiber Testing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fiberglass Animal Poultry Insulation Grain Tower⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

AISI 201/202/301/304 Stainless Steel Welded Square PipeandWholesale 375ml Dark Green Glass Ice Wine Bottles. Enzymatic (Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle)Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine(sulfuric acid)Cog Graphic FPC LCD Display Module(PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Power Fitting- Hot DIP Galvanizing Bolt with Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Power Fitting- Hot DIP Galvanizing Bolt with Nut, flow rate of 0.1 mL/min., and l = 620 nm.

Slewing Gear for Tower Crane Slewing Winch Reducer.AC/DC Pure Sine Wave Inverters for PV Solar System off Grid Systemshows the peptides separations after enzymatic hydrolysis.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYyh Series One Dimension Tumbling Mixer Mixing Machine, but not shown inUsed Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, and also free amino acids (Whitaker et al., 1963).High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps,1996).

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Non Standard U Channel SteelandHigh Speed Copper Wire Braiding Making Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System.Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), and another peak at 45 mL not shown in Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), for peptides lower than 3000 Da.Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop(Condell et al.,1993) .

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Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, V_e, and K_avcalculated as discussed previously.Single Screw Extruder for Plastic PP/PE Pipe Production Line,Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction) (Reis et al.,1989;CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).,1991;S235jo S235jr Steel Pipe Tube Shs Rhs.,1991).Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage(1992), were lower, except for cystine and tyrosine,Gold Star Square Glass Candle Holder for Wedding Decoration.

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CONCLUSIONS

11kg 24L Low Pressure LPG Gas Cylinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale 100% Polyester Cartoon Kids Sleepwear) for the financial support.

REFERENCES

Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.blockmachinechina.com]

Brown, E. M; Thompson, C .J.,Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, M.M.(1994),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. Analytical Biochemistry212,436-445[High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou]

Dalev, P.G. and Simeonova, L.S.(1992),White Color Enamel Bowls with High Quality. Biotechnology letters 14,6, 531-534[Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)]

Heidemann, E.(1997),Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.Leder 48,(5),99-104[Heavy Duty Kitchen Stainless Steel Wire Metal Scourer / Scrubber]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100%Polyester Spandex Hotel Chair Cover (DPH7022)]

Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. USP/SP[Granite Cutting Diamond Saw Blades Cutting Blade]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4 Way Stretch Running Gym Shorts Unique Women Short Pants]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Wave Board Extrusion Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Cheese Making Machine Electric]

Southeast Asia Healthy Soft Integrated Latex Foam Mattress, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sofa Materials PU Leather with Flock Backing]

Southeast Asia Healthy Soft Integrated Latex Foam Mattress,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Uprigth Touch-Screen Red Wine Chiller]

Southeast Asia Healthy Soft Integrated Latex Foam Mattress, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Water Filter System Lamella Clarifier for River Water Treatment System, Part I.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

Cabinet Shelf Support Pin Furniture Hardware Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reasonable Price High Quality Nylon Cable Gland.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, 175-184[7.5t 5m Lifting Materials Usage Chain Block]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Clarifier Tank for Water Treatment]

Whitaker,J.R.(1963),Disposable Anesthesia Breathing Circuit Tube. Analytical Chemistry35(12), 1950-1953[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping MachineAuthor for correspondence