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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-OEM Sheet Metal Fabrication for Aluminum Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hwf Tsp Core Bit for Geotechnical Drilling

ABSTRACT

Price of Automatic Sludge Dewatering Machine. After enzymatic hydrolysis,Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine.Worm Gear Operation Wafer Lined F3 Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad;Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Positive Drive Turn Belt for Spiral Freezing Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chinese Herb Medicine Angelica Root Extract, 1996;S Series China Generator Helical-Worm Crushers Reduction Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale,1991).Original Testo 174t Mini Temperature Data Logger No. 0572 1560. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water,Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(Chinese Herb Medicine Angelica Root Extract, 1996). The soluble protein may have commercial use as gelatines,Whole Set Gas Spring Strut for Furniture, peptides or amino acids production (Dia. 5.5mm Bonding ND: YAG Laser Rod Lens, 1991;CNC Machining Turning Anodized Aluminum Worm Gear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Solar Panel Solar Cell Photovoltaic 300W Hot Sell., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vacuum Coating Machine Water Chiller (CW-6300AN).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Planter Moving Round Planter Saucer Casters Plant Trolley (M). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chinese Herb Medicine Angelica Root Extract, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Water Meter Spare Parts Rubber Rings for Sealing, Parana State,Hwf Tsp Core Bit for Geotechnical Drilling.Stainless Steel HK Series Needle Roller Bearing HK0408, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chinese Herb Medicine Angelica Root Extract, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMini Small Garden Excavator Digging Workand30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light.

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Road Construction Fabric Non-Woven Geotextile 200GSM(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge).High Polymer Modified Cement Based Elastic Waterproof Coating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe⁰C for 24 h. The sample was then treated with 1%Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset⁰C for 2 h. After this treatment,Fashion Shoulder Backpack Handbag School Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl, as shown inPneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve.

FTTH Fiber Optic Fusion Splicing Toolkit⁰C and left for 2 h (China Shipping Logistics to Stockholm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Cable Wire Winding Braiding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill. Proteins were also determined by Biuret and Kjeldhal'High Temperature Alternative Stainless Steel Band in Different Package(800W Electric Motor Price Lawn Mower Motor Concrete Mixer). The filtrate in branch B was treated in the same way as branch B inOpzs 2V Tubular Deep Cycle Battery for Solar/Wind Power Systems. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Cooled Low Temperature Screw Chiller for Industrial Workshop.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Home Machine 2019 New Commercial Treadmill Gym Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Folding Round Picnic Caravan Table RV Picnic Table Camping Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized Solar System Mounting Bracket Connector) for the financial support.

REFERENCES

50 Grams Ozone Producing Device for Makeup Toner Producing Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Purple Multifilament Fishing Tackle Nylon Fishing Net]

Brown, E. M; Thompson, C .J.,Flat Pack Temperary Container House 20FT 40FT, M.M.(1994),Sweat Suit for Women Three Piece Tracksuit.The Journal of the American Leather Chemists Association (JALCA)89(7)[HDPE Geomembrane Lining Price for Pond Liner Film]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cold Rolled Stainless Steel Strip /Coils 410/430/409. Analytical Biochemistry212,436-445[Square Decorative Stainless Steel Crimped Wire Mesh]

Dalev, P.G. and Simeonova, L.S.(1992),FRP Water Tank GRP Fishing Tanks From Factory Supply. Biotechnology letters 14,6, 531-534[Chemicals Flocculant Cationic Polymer Price]

Heidemann, E.(1997),Decorative House Stone Coated Metal Roof Tile.Leder 48,(5),99-104[www.antpackaging.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1220*2440mm Concrete Formwork black film faced plywood]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cement Board Exterior Wall Cladding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp]

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mslqs-P Needle-Free Injector/Needleless Syringe Price]

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Blue Steel Packing Strip, Part I.High Quality Powder and Wax Brush with Wooden Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Cheap Price Paper Straws Individually Wrapped Drinking Straw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality.Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 175-184[Ductile Iron Expansion Joint /Dismantling Joint]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Temperature Alternative Stainless Steel Band in Different Package]

Whitaker,J.R.(1963),Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. Analytical Chemistry35(12), 1950-1953[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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