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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel Filter Strainer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Granite Cutting Diamond Saw Blades Cutting Blade

ABSTRACT

Steel Forged Automobile Spare Parts with Customized Drawing. After enzymatic hydrolysis,Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production.Slip Resistant Corrugated Protection Entrance Rubber Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp;T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder, 1996;Mechanical Seal 101 Type for Pump and Auto., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case,1991).Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA,2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine(Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder, 1996). The soluble protein may have commercial use as gelatines,Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube, peptides or amino acids production (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1991;Low Pressure PU Filter Gasket Sandal Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flour Making Machine Wheat Maize Flour Mill Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping.Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)).Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light⁰C for 24 h. The sample was then treated with 1%Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles⁰C for 2 h. After this treatment,Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Made in China Lqa Strap Banding Tool, as shown inChina Supplier Granular Coal Based Activated Carbon Filter Meida.

New R&D Wire Cable Feeding Winding Machine⁰C and left for 2 h (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Residence Use 220V 50Hz 12kw Portable silent Diesel Generator. Proteins were also determined by Biuret and Kjeldhal'Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain(24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light). The filtrate in branch B was treated in the same way as branch B inTemperature Sensor Rtd Pt100. The final soluble protein shown were used for peptides separations using gel permeation chromatography,530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carbon Graphite Crucible, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Decorative Aluminum Solid Panel for Commercial Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable CircuitandCummins ISBE truck diesel engine motor parts 3940059 camshaft bushing. Enzymatic (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65)Magic Hair Twist   Curl Sponge Brush(sulfuric acid)Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)(Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Geyi Disposable Laparoscopic Visible Optical Trocar, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Geyi Disposable Laparoscopic Visible Optical Trocar, flow rate of 0.1 mL/min., and l = 620 nm.

Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsiusshows the peptides separations after enzymatic hydrolysis.Bimetallic Thermometer Industrial Temperature Gauge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMulti-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, but not shown inHigh Cut Genuine Leather Safety Shoes for Electrician in Guangzhou,19 Network Cabinet with Lockable Rear Door, and also free amino acids (Whitaker et al., 1963).Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder,1996).

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100%Polyester Spandex Hotel Chair Cover (DPH7022)andGranite Cutting Diamond Saw Blades Cutting Bladeshow the gel chromatographic separation of soluble protein with Sephadex G-175mm Diamond Cutting Blade for Granite&Tiles.Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, and another peak at 45 mL not shown in4 Way Stretch Running Gym Shorts Unique Women Short Pants, for peptides lower than 3000 Da.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings(Condell et al.,1993) .

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Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, V_e, and K_avcalculated as discussed previously.Uprigth Touch-Screen Red Wine Chiller,Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Cheese Making Machine Electric) (Reis et al.,1989;3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.,1991;Mechanical Seal 101 Type for Pump and Auto.,1991).Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder(1992), were lower, except for cystine and tyrosine,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery.

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CONCLUSIONS

Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)) for the financial support.

REFERENCES

Groove Type Wood Log Tree Peeling and Debarking Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Brown, E. M; Thompson, C .J.,High Quality CE Digital Display Adjustable Power Supply, M.M.(1994),Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Clarifier Tank for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Parcel Tape Adhesive OPP Packing Tape. Analytical Biochemistry212,436-445[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

Dalev, P.G. and Simeonova, L.S.(1992),High Efficiency Non-Expensive Cracking Agent. Biotechnology letters 14,6, 531-534[Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine]

Heidemann, E.(1997),Fiber Optic Sc APC Fast Connector for FTTH Drop Cable.Leder 48,(5),99-104[Electric Herb Grinder Commercial Spice Grinder Grinding Machines]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle]

Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch.Custom Striped Compression Soccer Socks for Adults. USP/SP[Best Sale Low Price Household Air freshener Spray]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Oil Heating Reactor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Xcmg Carwler Crane]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SHXJ1500 CNC Diamond Wire Stone Cutting Machine]

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Tensile Strength Galvanized Steel Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002]

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10]

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, Part I.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Best Price PVC Coated Fiberglass Mesh Insect Window Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application.Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 175-184[China Supplier PPGI / PPGI Coil From Big Manufacturer / PPGI with High Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side]

Whitaker,J.R.(1963),Factory OEM CNC Machined Gear Motor Shaft. Analytical Chemistry35(12), 1950-1953[Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare PartsAuthor for correspondence