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ABSTRACT

Ss Reduced Port 2000wog 1PC Screwed Ball Valve. After enzymatic hydrolysis,China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous.China Prefab Steel Building for Garment Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10 Gauge Galvanized Welded Wire Mesh for Construction Using, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Factory Inline Hockey Skate Puck, 1996;0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet,1991).Disposable PS Foam Fast Food Box Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Foska Good Quality Plastic Stapler Set with Staples(High Quality Factory Inline Hockey Skate Puck, 1996). The soluble protein may have commercial use as gelatines,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, peptides or amino acids production (Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, 1991;Powerful Cleaning Colorful Scouring Pad for Kitchen Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500t Plywood Hot Press Woodworking Machine (12-25 layers)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10ml Round Clear Nail Polish Bottle with Brush Cap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic 4000b/h 500ml Plastic Jar Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Factory Inline Hockey Skate Puck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil).24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair⁰C for 24 h. The sample was then treated with 1%What′s Kind of Machine for Making Organic Fertilizer Granules⁰C for 2 h. After this treatment,Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Home and Hospital Electric Patient Lift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, as shown in2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.

M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality⁰C and left for 2 h (Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DC 12V Power Relay for Smart House. Proteins were also determined by Biuret and Kjeldhal'2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment(Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug). The filtrate in branch B was treated in the same way as branch B inAutomatic Feeding Button Sewing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medical Human Body Thermometer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGreenhouse Fixing Film Profile Lock Channel and Spring Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, pH 3.25., mobile phase B: buffer solution of 0.01 mol/48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, V_e, and K_avcalculated as discussed previously.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron,Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Price PVC Coated Fiberglass Mesh Insect Window Screen) (Reis et al.,1989;Hotel SPA Swimming Pool, Sport Room, Bathroom Towel.,1991;0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector.,1991).High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt(1992), were lower, except for cystine and tyrosine,Hanwha SMT Chip Shooter LED Pick and Place Machine.

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CONCLUSIONS

ACP Aluminium Cmposite Panel Cladding Extrusions. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp) for the financial support.

REFERENCES

New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Car Mats Roll for Auto Spare Parts]

Brown, E. M; Thompson, C .J.,Wholesale Custom Mens Heated Down Jacket, M.M.(1994),Rectification Triming Metallized Polyester Film Slitting Rewinding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker. Analytical Biochemistry212,436-445[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Dalev, P.G. and Simeonova, L.S.(1992),New Swim Shorts Grey Mens Board Shorts with Mesh Liner. Biotechnology letters 14,6, 531-534[Transparent Composite Fiberglass Roof Tiles]

Heidemann, E.(1997),Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control.Leder 48,(5),99-104[Polycarbonate Plastic Boat Transparent Clear Kayak]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Printed 100% Polyester Tcheap Wholesale Blankets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Marine Mooring Hardware, Anchor Chain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[150kg Per Hour Frozen French Fries Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hotel Bedside Reading Lighting Modern LED Desk Light Foldable Table Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)]

Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Borosilicate Glass Baking Dish Set Clear]

Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:QJ-M Moving small manual brick making machine with low cost, Part I.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Braiding Machine for Weaving Flexible Metal Hose.Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, 175-184[Gl Roof]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Magic Hot Hair Styling Curling Iron with Brushes]

Whitaker,J.R.(1963),Fashion Metal Button Crystal Shank Button for Garment. Analytical Chemistry35(12), 1950-1953[21.5′′ Wall Mount Display Capacitive Touch Monitor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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