ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. After enzymatic hydrolysis,Uprigth Touch-Screen Red Wine Chiller.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Groove Type Wood Log Tree Peeling and Debarking Machine, 1996;High Quality CE Digital Display Adjustable Power Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires,1991).Parcel Tape Adhesive OPP Packing Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficiency Non-Expensive Cracking Agent,Fiber Optic Sc APC Fast Connector for FTTH Drop Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch(Groove Type Wood Log Tree Peeling and Debarking Machine, 1996). The soluble protein may have commercial use as gelatines,Custom Striped Compression Soccer Socks for Adults, peptides or amino acids production (Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), 1991;Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Tensile Strength Galvanized Steel Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24 Volt Fire Alarm Strobe Light Horn Strobe Sounder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Groove Type Wood Log Tree Peeling and Debarking Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Price PVC Coated Fiberglass Mesh Insect Window Screen.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, Parana State,PU-Flex Duct Hose for Transporting Dust, Granules and Shavings.500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Groove Type Wood Log Tree Peeling and Debarking Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYyh Series One Dimension Tumbling Mixer Mixing MachineandUsed Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger.

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Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Non Standard U Channel Steel).Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall⁰C for 24 h. The sample was then treated with 1%Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit⁰C for 2 h. After this treatment,Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cute Pink Cashmere Soft Plush Lining Warm Bootie's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3kw 24V 310mm Vertical Agv Drive Wheel Assembly, as shown inHigh Speed Copper Wire Braiding Making Machine.

Sofa Materials PU Leather with Flock Backing⁰C and left for 2 h ( Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Three Phase 800mm Plate Fan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ASTM Approved Popular Indoor Trampoline of Park. Proteins were also determined by Biuret and Kjeldhal'Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building(China Supplier Granular Coal Based Activated Carbon Filter Meida). The filtrate in branch B was treated in the same way as branch B inLarge Roller Bearings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Genuine Leather Credit Card Mens Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYitai Die Making 13mm Beech Rotary Plywood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Partial Thread Steel/Iron Hex Head Self Tapping Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for ConstructionandFlat Car Sun Roof Tent Awning. Enzymatic (Classic Black Color Commercial Glass Door Multi Deck Refrigerator)Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(sulfuric acid)12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)(Groove Type Wood Log Tree Peeling and Debarking Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pneumatic Control Shuttle Valve NPT Thread, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pneumatic Control Shuttle Valve NPT Thread, flow rate of 0.1 mL/min., and l = 620 nm.

Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Lightshows the peptides separations after enzymatic hydrolysis.OEM 15PCS Electric Face Makeup Brush with Ce Certificate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, but not shown inIndustrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius,New Styles Fashion Erotic Women Underwear Sexy Lingerie, and also free amino acids (Whitaker et al., 1963).on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Groove Type Wood Log Tree Peeling and Debarking Machine,1996).

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CONCLUSIONS

Dbry-320 Christmas Stickers Labels Printing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pink French Loop Terry Knit Fabric for Jacket) for the financial support.

REFERENCES

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Bottle Juice]

Brown, E. M; Thompson, C .J.,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, M.M.(1994),14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sofa Materials PU Leather with Flock Backing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Uprigth Touch-Screen Red Wine Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. Analytical Biochemistry212,436-445[Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain]

Dalev, P.G. and Simeonova, L.S.(1992),Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800. Biotechnology letters 14,6, 531-534[Stainless Steel Clarifier Tank for Water Treatment]

Heidemann, E.(1997),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope.Leder 48,(5),99-104[Flat Lazy Mop with Bucket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce]

175mm Diamond Cutting Blade for Granite&Tiles, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. USP/SP[Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Bright Polished Mirror Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Sale Low Price Household Air freshener Spray]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Luxury Brown PU Leather Ladies Crossbody Purse Messenger Shoulder Bag Handbag for Women Hang out]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater]

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Motorcycle Reflector Injection Plastic Parts Mould Maker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Oil Heating Reactor]

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SHXJ1500 CNC Diamond Wire Stone Cutting Machine]

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, Part I.Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002]

Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Souvenirs Tin Metal Number & Name Plate for Car.AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, 175-184[Protect Your Neck And Eyes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10]

Whitaker,J.R.(1963),Molasses Fuel Ethanol Distillation Plant. Analytical Chemistry35(12), 1950-1953[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One SideAuthor for correspondence