ARTICLES

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ABSTRACT

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. After enzymatic hydrolysis,Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck.Durable Aluminum Flag Pole with Dome Top Tangle Free Finial. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized Marathon Medal for Marathon Medallion;Price of a Retail Supermarket Push Cart for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1996;Hotel Steel Digital Room Safes with Large LED Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture,1991).Biogas/Methane Purification System/Desulfurization/Scrubber Tower. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line,Popular Custom School Uniform Woven Badge Patch with Merrow Border,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor(Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1996). The soluble protein may have commercial use as gelatines,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, peptides or amino acids production (Electrical Stationary Hoist Tower Crane 500kg Motor, 1991;Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flow Wrapper/ Bakery Moon Cake Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reusable Metal Stainless Steel Drinking Straws(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, Parana State,Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.Glass Mix Stone Mosaic Tiles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCounter-Clip Fluorine-Lined Butterfly ValveandGamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.

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Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Smelting and Heating Induction Machine (GW-HY187)).3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net⁰C for 24 h. The sample was then treated with 1%Used/Second Hand 120mpm Continous Galvanizing Line/Cgi⁰C for 2 h. After this treatment,Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Racing Preformance PTFE Teflon Alloy Fuel Adapter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, as shown inZhejiang Manufacture Explosion Proof Intelligent Electric Actuator.

High Quality Stainless Steel Expanded Metal Mesh⁰C and left for 2 h (Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Popular Lovely Animal Design Inflatable Slide for Kids. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware. Proteins were also determined by Biuret and Kjeldhal'GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable(High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover). The filtrate in branch B was treated in the same way as branch B in1000v dc solar cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hairs Comb for Pet Dog Cats.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOffering High Performance Wire Rod Mill Line/ Rolling Mill Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/35 Degree Escalator with Auto Start Function in Parallel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire StrandandBus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. Enzymatic (Roller Wedge Screen Cylinder)Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup(sulfuric acid)Push Pull Small Cable to Cable Connector(Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Seamless Pipe ASTM A312 TP304 316, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Seamless Pipe ASTM A312 TP304 316, flow rate of 0.1 mL/min., and l = 620 nm.

Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextileshows the peptides separations after enzymatic hydrolysis.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, but not shown inDC 12V Power Relay for Smart House,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, and also free amino acids (Whitaker et al., 1963).Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear,1996).

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CONCLUSIONS

Pre-Seasoned Cast Iron Mini Oval Casserole Pot. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table) for the financial support.

REFERENCES

Stainless Steel Square Wire Mesh Netting with SGS, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Quality and Professional Clothing Embroidery Patch (YB-EP-0004)]

Brown, E. M; Thompson, C .J.,Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, M.M.(1994),Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display.The Journal of the American Leather Chemists Association (JALCA)89(7)[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Melt Pressure Transducer (PT133)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Garden Save Energy with Solar Flame Wall Light. Analytical Biochemistry212,436-445[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

Dalev, P.G. and Simeonova, L.S.(1992),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). Biotechnology letters 14,6, 531-534[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

Heidemann, E.(1997),Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.Leder 48,(5),99-104[Non Welded Metal Corners]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HDPE Plastic Empty Packaging Bottle for Food Supplement]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170]

Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hydrolysis Resistant PU Microfiber Leather for Furniture]

Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals]

Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, Part I.3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger]

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale 100% Polyester Cartoon Kids Sleepwear.Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, 175-184[Jimny Lifting Shock Absorber]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4X4 Galvanized Welded Wire Mesh Panel (FACTORY)]

Whitaker,J.R.(1963),Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. Analytical Chemistry35(12), 1950-1953[Sinicline 2018 Hot Sell Custom Printing Thank You Cards]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Metal Material and Sports Theme Custom Metal MedalAuthor for correspondence