ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Push Pull Small Cable to Cable Connector. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Seamless Pipe ASTM A312 TP304 316

ABSTRACT

Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. After enzymatic hydrolysis,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars.Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce;Asco 353 Series 3/4 Pulse Valve Solenoid Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1996;Purple UL 1095 16AWG Electronic Lead Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Brushed Anodizing Aluminum Extrusion Profile for LED,1991).PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,S235jo S235jr Steel Pipe Tube Shs Rhs,Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre-Seasoned Cast Iron Mini Oval Casserole Pot(Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Square Wire Mesh Netting with SGS, peptides or amino acids production (Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, 1991;Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Garden Save Energy with Solar Flame Wall Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length.Non Stick Silicone Coated Fiberglass Cloth-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, Parana State,Stainless Steel Seamless Pipe ASTM A312 TP304 316.Hot Sale 100% Polyester Cartoon Kids Sleepwear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the15mm Best Price Commercial Grade Birch Plywood for FurnitureandGsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine.

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Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet).Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Laparoscopy Clip Applicator for Abdominal Surgery Uses⁰C for 24 h. The sample was then treated with 1%High Yield Tree Stump Crusher Machine⁰C for 2 h. After this treatment,Diaphragm Valve for Bag Filter Since 1992. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, as shown inASTM A240 309s Stainless Steel Sheet.

Manufacturer Plastic Injection Mould Molding Nylon PP Injection⁰C and left for 2 h (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack. Proteins were also determined by Biuret and Kjeldhal'Aluminum Forging Cast Metal Sand Die Casting(Chinese 1325 Ce Certificated CNC Engraving Router for Sale). The filtrate in branch B was treated in the same way as branch B inCereal Bar Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ADC12 Aluminum Die Casting Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Logo Cotton Terry Oversize Pullover Men Hoodie(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PVC Fabric Rapid Roller Shutter for Clearn Room, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Truck Auto New Round Offroad Laser 7 Inch LED Driving LightsandFiberglass Animal Poultry Insulation Grain Tower. Enzymatic (Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon)Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(sulfuric acid)Wholesale 375ml Dark Green Glass Ice Wine Bottles(Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine.Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leashshows the peptides separations after enzymatic hydrolysis.Cog Graphic FPC LCD Display Module(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Apparel Applique Round Patch School Club Embroidery Collar Patch, but not shown inHeavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch,Power Fitting- Hot DIP Galvanizing Bolt with Nut, and also free amino acids (Whitaker et al., 1963).Slewing Gear for Tower Crane Slewing Winch Reducer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle,1996).

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Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive ProductionandCopy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, and another peak at 45 mL not shown inMen Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, for peptides lower than 3000 Da.High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(Condell et al.,1993) .

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Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System, V_e, and K_avcalculated as discussed previously.Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8),Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mini Small Garden Excavator Digging Work) (Reis et al.,1989;Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter.,1991;Purple UL 1095 16AWG Electronic Lead Wire.,1991).Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved(1992), were lower, except for cystine and tyrosine,Single Screw Extruder for Plastic PP/PE Pipe Production Line.

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CONCLUSIONS

Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge) for the financial support.

REFERENCES

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2.2 Ton Mini Hydraulic Excavator Machine for Sale From Mini Digger Manufacturer]

Brown, E. M; Thompson, C .J.,Gold Star Square Glass Candle Holder for Wedding Decoration, M.M.(1994),11kg 24L Low Pressure LPG Gas Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Analytical Biochemistry212,436-445[China Shipping Logistics to Stockholm]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop. Biotechnology letters 14,6, 531-534[800W Electric Motor Price Lawn Mower Motor Concrete Mixer]

Heidemann, E.(1997),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.Leder 48,(5),99-104[Facial Skin Analysis Wrinkle Pigment Lesion Analyzer Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Compression Polyester Light Ankle Support Brace for Youth]

Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Precision Customized OEM Auto Hardware Lathe CNC Machining Parts.White Color Enamel Bowls with High Quality. USP/SP[ 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Road Construction Fabric Non-Woven Geotextile 200GSM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Geyi Disposable Laparoscopic Visible Optical Trocar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Custom Carbon Steel White Zinc Cross Recessed Hex Bolt and Nut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Advanced Unlocking Tool for Locksmith]

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China]

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top Great Wallet Genuine Leather Purse with Coin Pocket]

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, Part I.Southeast Asia Healthy Soft Integrated Latex Foam Mattress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header]

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Filter System Lamella Clarifier for River Water Treatment System.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), 175-184[Weld Neck Reducing Flange]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Sheet Metal Fabrication for Aluminum Parts]

Whitaker,J.R.(1963),Cabinet Shelf Support Pin Furniture Hardware Fittings. Analytical Chemistry35(12), 1950-1953[Used/Second Hand 120mpm Continous Galvanizing Line/Cgi]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cabinet Shelf Support Pin Furniture Hardware FittingsAuthor for correspondence