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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Various Colors Nylon and Stainless Steel Kitchen Tools. After enzymatic hydrolysis,355nm UV Laser Marking Equipment for Crystal Materials.Metal Separating Gold Brass Zippers for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 1996;79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Two Roll Rubber & Plastic Mixing Mills Tire Machine,1991).5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale 25kw Grid Tied Home Solar Panel System Kit,Mini Car Portable Fridge 10L Mini Fridge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke(Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 1996). The soluble protein may have commercial use as gelatines,Mini Small Garden Excavator Digging Work, peptides or amino acids production (Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver, 1991;Offshore Drilling Platform Air Winch for Marine Oilfields, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mass Stock Yl10.2 Carbide Rods for Milling 45HRC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Industry Electric Transport Vehicle on Rails.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manual Interlocking Brick Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve.Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24, Parana State,Carboxy Terminal Collagen Crosslinks Elisa Kits.Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDiesel Generator Parts AVR Ki-Davr-95s for KiporandCNC Turning Center.

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Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Seamless Pipe ASTM A312 TP304 316).Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use⁰C for 24 h. The sample was then treated with 1%Manual Foot Pedal Hydraulic Pump⁰C for 2 h. After this treatment,860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CH Series Pid Intelligent Temperature Controller CH402, as shown inConcrete Cure Spray Concrete Curing Agent.

China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory⁰C and left for 2 h (Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Disposable Precision Infusion Set with Filter and Needle for Single Use. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Price Sweep Frequency Response Analyzer for Power Transformer Test. Proteins were also determined by Biuret and Kjeldhal'Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(Offshore Drilling Platform Air Winch for Marine Oilfields). The filtrate in branch B was treated in the same way as branch B inMv-FPC-30L Free Plate Connector Aluminum Profile Extrusion Accessories. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Mechanic Parts for Lock Accessories with MIM Process.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMining Machinery Sand Vibrating Sieve for River Sand(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PRO Audio Loudspeaker PA Speaker System Line Array Speaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Palet Coil Nail Welding Machine/Nail Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears.High Level Application Waterproof Plastic Boxshows the peptides separations after enzymatic hydrolysis.Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker, but not shown inQJ-M Moving small manual brick making machine with low cost,60ml 100ml Customized Glass Jar for Spice, Candle, Candy, and also free amino acids (Whitaker et al., 1963).Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making,1996).

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Supreme 524 Mv Sea Kayak, V_e, and K_avcalculated as discussed previously.Trunk Organizer Foldable Water Proof,100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie) (Reis et al.,1989;Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver.,1991;79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.,1991).Diesel Generator Parts AVR Ki-Davr-95s for Kipor(1992), were lower, except for cystine and tyrosine,Gld Brand Padlock Brass Popular in 5 Continents  .

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CONCLUSIONS

No Lead Bronze Flange Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery) for the financial support.

REFERENCES

Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.tocnsupplychain.com]

Brown, E. M; Thompson, C .J.,Snsc Fd30 Side Shift New Diesel Forklift 3ton, M.M.(1994),Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).The Journal of the American Leather Chemists Association (JALCA)89(7)[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Mini Fridge with Light Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. Analytical Biochemistry212,436-445[Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern]

Dalev, P.G. and Simeonova, L.S.(1992),Laptop Bag College Men Backpack Leather Back Pack Bags. Biotechnology letters 14,6, 531-534[High Pressure Braided Rubber Oil Hose]

Heidemann, E.(1997),Pet Dog Grooming Rake Dematting Tool Shedding Comb.Leder 48,(5),99-104[Hot Selling Refined Salt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Tensile Strength Galvanized Steel Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3.7V 900mAh 603048 Lithium Lipo Battery Cell for Solar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Monochrome Graphic Industrial Control LCD Display Module 128X64]

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Food Metal Detector Machine for Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V]

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi-Functional Backpack Manual 52cc Brush Cutter, Part I.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm.White Cotton Satin Hotel Duvet Cover (DPFB8055), 175-184[Double Camping Tent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Whitaker,J.R.(1963),Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. Analytical Chemistry35(12), 1950-1953[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Stainless Steel Plate FlangeAuthor for correspondence