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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-YAMAHA Yv100II Nozzle Horder Square Head Short Type. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Juice Doypack Packing Machine (ZLDB-4)

ABSTRACT

Stainless Steel Wok with Soft Touch Handle. After enzymatic hydrolysis,Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa.Funny Newest Design for Children Theme Outdoor Playground Park. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter;Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, 1996;Good Strength GRP Cooling Towers for Mechanical Cooling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash,1991).Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts,Loquat Juice and Loquat Syrup Production Machinery Export Worldwide,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material(Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, 1996). The soluble protein may have commercial use as gelatines,Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, peptides or amino acids production (Concrete Floor Grinding Disc Machine for Four Segments, 1991;Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Garden Furniture Rattan Sofa Set with Ottomen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Non Standard U Channel Steel.Socket Flange-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dn32 (11/4) BSPT Thread Air Actuated Piston Valves(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer).CNC 3 Axis Milling Machining Center(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting⁰C for 24 h. The sample was then treated with 1%Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter⁰C for 2 h. After this treatment,4 Axis CNC Woodworking Machinery Engraver Router. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes, as shown in100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale.

Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216⁰C and left for 2 h (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pre-Seasoned Cast Iron Baking Stone Cookware. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Compression Polyester Light Ankle Support Brace for Youth. Proteins were also determined by Biuret and Kjeldhal'Good Quality White PVC Rigid Foam Board for Iran Market(Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge). The filtrate in branch B was treated in the same way as branch B inBrush Cleaner Egg. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Precision Piston Ring Good Shape Ability High Quality Surface(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Farm Relief Plastic Automatic Air Release Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover.As568 Standard Brown FKM Rubber O Ring for Sealingshows the peptides separations after enzymatic hydrolysis.Multi Color Spade Made of Steel and Panting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed Roll Plastic T-Shirt Bag Making Machine, but not shown inSand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, and also free amino acids (Whitaker et al., 1963).Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card,1996).

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CONCLUSIONS

Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Sterile Twist Top Blood Lancet) for the financial support.

REFERENCES

100W 120W 150W Outdoor Solar LED Street Lighting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[19mm 1no Black Momentary Flat Metal Push Button Switch with Screw Terminal]

Brown, E. M; Thompson, C .J.,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), M.M.(1994),Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Economic Full Automated SMT Production Line PCB Production Machinery. Analytical Biochemistry212,436-445[Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. Biotechnology letters 14,6, 531-534[Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug]

Heidemann, E.(1997),Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket.Leder 48,(5),99-104[Webest Package Creative Sky Blue Special Paper Jewelry Box Custom Jewellery Box in Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder]

Mslqs-P Needle-Free Injector/Needleless Syringe Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206).Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. USP/SP[Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Linear Guideway for CNC Machine as Components]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HDPE Geomembrane Lining Price for Pond Liner Film: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[jinbinvalve.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Pressure PU Filter Gasket Sandal Making Machine]

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Varied Colored As568 Food Grade Soft Rubber Silicone O Ring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter]

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, Part I.Chinese Galvanized Rectangular Tube for Buildings Materials. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

China Customized Chilled Cast Iron Roll, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),IMTEK High Precision and Rigidity Linear Guides for CNC machinery.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask, 175-184[Hot Selling Reusable Pasties Adhesive Silicone Girls Sexy Nipple Covers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Galvanized Wave Form Perforated Steel Strips]

Whitaker,J.R.(1963),Yashi Touch Screen Smart Interactive Whiteboard for Office. Analytical Chemistry35(12), 1950-1953[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete PumpAuthor for correspondence