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ABSTRACT

Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. After enzymatic hydrolysis,Custom Striped Compression Soccer Socks for Adults.Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen;High Tensile Strength Galvanized Steel Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, 1996;Best Price PVC Coated Fiberglass Mesh Insect Window Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application,1991).Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory OEM CNC Machined Gear Motor Shaft,High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pink French Loop Terry Knit Fabric for Jacket(from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, 1996). The soluble protein may have commercial use as gelatines,Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, peptides or amino acids production (Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, 1991;500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cute Pink Cashmere Soft Plush Lining Warm Bootie(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Genuine Leather Credit Card Mens Wallet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming).Yitai Die Making 13mm Beech Rotary Plywood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gear Rolling Machine Zh28/25 Spline Hobbing Machine⁰C for 24 h. The sample was then treated with 1%OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts⁰C for 2 h. After this treatment,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Partial Thread Steel/Iron Hex Head Self Tapping Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flat Car Sun Roof Tent Awning, as shown inDn32 (11/4) BSPT Thread Air Actuated Piston Valves.

Classic Black Color Commercial Glass Door Multi Deck Refrigerator⁰C and left for 2 h (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026). Proteins were also determined by Biuret and Kjeldhal'Pneumatic Control Shuttle Valve NPT Thread(Chicken Cage Mesh Hexagonal Wire Mesh Machine). The filtrate in branch B was treated in the same way as branch B inChina Second Hand Dump Truck. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Styles Fashion Erotic Women Underwear Sexy Lingerie(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stand Hot and Cold Normal Water Dispenser with Refrigerator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Construction Welding Rods Electrode E6013 E7018 E7016, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dbry-320 Christmas Stickers Labels Printing Machine.Roller Wedge Screen Cylindershows the peptides separations after enzymatic hydrolysis.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFreshwater Pearl Butterfly Flower Bride Hairpin, but not shown inBoho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, and also free amino acids (Whitaker et al., 1963).14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder,1996).

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High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, V_e, and K_avcalculated as discussed previously.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck,Aluminum Bright Polished Mirror Sheet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles) (Reis et al.,1989;Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts.,1991;Best Price PVC Coated Fiberglass Mesh Insect Window Screen.,1991).Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)(1992), were lower, except for cystine and tyrosine,Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.

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CONCLUSIONS

O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Three Phase 800mm Plate Fan) for the financial support.

REFERENCES

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beam Spot Wash 3in1 Moving Head]

Brown, E. M; Thompson, C .J.,Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, M.M.(1994),Customized Souvenirs Tin Metal Number & Name Plate for Car.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. Analytical Biochemistry212,436-445[Designed Steel Frame Factory Building]

Dalev, P.G. and Simeonova, L.S.(1992),Molasses Fuel Ethanol Distillation Plant. Biotechnology letters 14,6, 531-534[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Heidemann, E.(1997),Pond Self Priming Booster Pressure Water Pump with Check Valve.Leder 48,(5),99-104[Steel Build]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[By6-8-200-L Automatic Doypack Premade Pouch Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Customized Chilled Cast Iron Roll]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Slide for Indoor Play Structure]

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Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tct Circular Saw Blade for Grass Cutting]

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Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1jo4 Hydraulic Adaptors for Hose Fittings.Chinese 1325 Ce Certificated CNC Engraving Router for Sale, 175-184[Vof Fhsm-705 Multi-Purpose Single Needle Household Jeans Overlock Household Maquinas De Coser Sewing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)]

Whitaker,J.R.(1963),Oil Heating Reactor. Analytical Chemistry35(12), 1950-1953[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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