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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-E171 Standrad Titanium Dioxide Food Grade A200. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Elbow Forming Machine Gorelocker HVAC Duct Making Machine

ABSTRACT

Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh. After enzymatic hydrolysis,Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses.L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric;304 Stainless Steel Anti-Corrosion Welded Wire Mesh; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Made Colorful Sublimation Womens Yoga Pants Wholesale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, 1996;Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Fashion PVC Sequins Adjustable Women Brace Suspenders,1991).Solid Flex-Drain Pipe Length 40′. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator,in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500(Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, 1996). The soluble protein may have commercial use as gelatines,W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/, peptides or amino acids production (Cake/Cookies/Bread Automatic Package and Packing Machine, 1991;Customize Milky Extrusion PC Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;European Good Quality Bathroom Simple Sliding Glass Shower Cabina., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fruit Plum Cleaning Washing Machine with Brushes Spray. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector.11.1V 2000mAh Lithium Polymer Battery Pack Customized Size-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FTTH Fiber Optic Fusion Splicing Toolkit).Slip Resistant Corrugated Protection Entrance Rubber Floor Mat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp⁰C for 24 h. The sample was then treated with 1%T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)⁰C for 2 h. After this treatment,High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mechanical Seal 101 Type for Pump and Auto, as shown inSafe Durable Scaffolding Trapdoor Plank for Construction.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flour Making Machine Wheat Maize Flour Mill Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Small Cleaning Brush ESD Anti Static Brush ESD Brush BlackandFactory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping. Enzymatic (Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh)Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment(sulfuric acid)Metal Material and Sports Theme Custom Metal Medal(Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, flow rate of 0.1 mL/min., and l = 620 nm.

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3).New R&D Wire Cable Feeding Winding Machineshows the peptides separations after enzymatic hydrolysis.Full Computer Plain Socks Knitting Machine with High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, but not shown inGYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, and also free amino acids (Whitaker et al., 1963).New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter,1996).

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CONCLUSIONS

Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers) for the financial support.

REFERENCES

Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fasoracetam Choline]

Brown, E. M; Thompson, C .J.,Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain, M.M.(1994),530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. Analytical Biochemistry212,436-445[Hotel Steel Digital Room Safes with Large LED Display]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Logo Cotton Terry Oversize Pullover Men Hoodie]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Aluminum Cover Frame Half Aluminum Cover Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Decorative Aluminum Solid Panel for Commercial Building: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0280156318 Fuel Injector for Peugeot Citroen 1.6L High Impedance]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbon Graphite Crucible]

Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Di DIN3352 Double Orifice Automatic Air Release Valve Dn65-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil]

Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle]

Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Magic Hair Twist   Curl Sponge Brush, Part I.Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP]

Geyi Disposable Laparoscopic Visible Optical Trocar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.Bimetallic Thermometer Industrial Temperature Gauge, 175-184[Cnc Milling Machine Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug]

Whitaker,J.R.(1963),19 Network Cabinet with Lockable Rear Door. Analytical Chemistry35(12), 1950-1953[Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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