ARTICLES

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ABSTRACT

Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling. After enzymatic hydrolysis,Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts.100W 120W 150W Outdoor Solar LED Street Lighting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901);Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Economic Full Automated SMT Production Line PCB Production Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1996;Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206),1991).Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,HDPE Geomembrane Lining Price for Pond Liner Film,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Varied Colored As568 Food Grade Soft Rubber Silicone O Ring(High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1996). The soluble protein may have commercial use as gelatines,2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, peptides or amino acids production (Chinese Galvanized Rectangular Tube for Buildings Materials, 1991;China Customized Chilled Cast Iron Roll, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IMTEK High Precision and Rigidity Linear Guides for CNC machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yashi Touch Screen Smart Interactive Whiteboard for Office. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Fireplace PCBA with Double Layers Circuit Board.China Shipping Logistics to Stockholm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Yc Pure White Nylon Powder for 3D Printing, Parana State,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering ScreenandGood Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.

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Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches).High Quality Liquid Spouted Stand up Pouch with Custom Logo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container⁰C for 24 h. The sample was then treated with 1%Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel⁰C for 2 h. After this treatment,China Modern Pharmacy Vacuum Filter Press. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Cotton Luxury Printed Bedding Comforter Set's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mouse Pad with Gel Wrist Rest, as shown inBest Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses.

Custom Size Christmas Gift Personalized Watch Boxes for Men⁰C and left for 2 h (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Selling Quality Aluminum Acetylene Gas Cylinder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coal Mine Mini Electric Excavator with Quick Coupling. Proteins were also determined by Biuret and Kjeldhal'2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank(Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone). The filtrate in branch B was treated in the same way as branch B infrom China to Korea Jeju reliable low cost Air Logistics Freight Shipping Forwarder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Grade Transparent Cookie Airtight Plastic Container Jars.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBoho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2 Round Sash Pulley for Chain - Brass (PVD)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anodized Aluminum Expanded Metal Mesh Competitive Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot DIP Galvanized G2150 D Ring Shackle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pneumatic Control Shuttle Valve NPT ThreadandOffice Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. Enzymatic (Round Shape Aluminum PCB and PCBA)36PCS LED Gold Matrix Stage Zoom Light(sulfuric acid)Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China(High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chicken Cage Mesh Hexagonal Wire Mesh Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chicken Cage Mesh Hexagonal Wire Mesh Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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Chloroacetic Acid Merck

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CONCLUSIONS

Chinese Supplier of PVC Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Animal Agricultural Damping Horse Stall Rubber Cow Mat) for the financial support.

REFERENCES

Tianjin Manufacturer PTFE Coated Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashion Jewelry Hip Hop 18K 24K Gold Diamond Gem Stone Pendant Necklace]

Brown, E. M; Thompson, C .J.,Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, M.M.(1994),Wholesale Prices of 20W 40W 60W Solar Street Lights.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sinicline Custom Brand with Plastic Pouch Hangtag for Hats. Analytical Biochemistry212,436-445[ABC-508 Brake System Cleaning and Fluid Exchanging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder. Biotechnology letters 14,6, 531-534[Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)]

Heidemann, E.(1997),Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag.Leder 48,(5),99-104[Metal Running Medal Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Slip Resistant Corrugated Protection Entrance Rubber Floor Mat]

6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A).ALS Stage 5W RGB Animation Laser Light. USP/SP[CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Separating Gold Brass Zippers for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Playground Slide]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)]

Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pet Anti-Static Needled Mat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts]

Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dia. 5.5mm Bonding ND: YAG Laser Rod Lens]

Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, Part I.OEM Sheet Metal Fabrication for Aluminum Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

Hwf Tsp Core Bit for Geotechnical Drilling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Price of Automatic Sludge Dewatering Machine.Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, 175-184[Canada Light Steel House Ceiling Channel Keel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

Whitaker,J.R.(1963),Worm Gear Operation Wafer Lined F3 Butterfly Valve. Analytical Chemistry35(12), 1950-1953[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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