ARTICLES

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ABSTRACT

Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator. After enzymatic hydrolysis,PVC Cabinet Construction Board, White 18mm PVC Foam Board.2019 New Portable Mini Dental X-ray Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ss Reduced Port 2000wog 1PC Screwed Ball Valve;China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Prefab Steel Building for Garment Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, 1996;Cable Tray with ′t′ Slots., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10 Gauge Galvanized Welded Wire Mesh for Construction Using,1991).High Quality Factory Inline Hockey Skate Puck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable PS Foam Fast Food Box Making Machine(Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, 1996). The soluble protein may have commercial use as gelatines,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), peptides or amino acids production (Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, 1991;Foska Good Quality Plastic Stapler Set with Staples, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hotel SPA Swimming Pool, Sport Room, Bathroom Towel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powerful Cleaning Colorful Scouring Pad for Kitchen Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500t Plywood Hot Press Woodworking Machine (12-25 layers).10ml Round Clear Nail Polish Bottle with Brush Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic 4000b/h 500ml Plastic Jar Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag).Accept OEM New Products Team Various Colors Gold Ribbon(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut⁰C for 24 h. The sample was then treated with 1%Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh⁰C for 2 h. After this treatment,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)What′s Kind of Machine for Making Organic Fertilizer Granules, as shown inCustom Made Colorful Sublimation Womens Yoga Pants Wholesale.

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine⁰C and left for 2 h (Automatic French Fries Frying Machine with Low Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Home and Hospital Electric Patient Lift. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. Proteins were also determined by Biuret and Kjeldhal'M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality(3.5ton Chinese Engine Gasoline Forklift (FG35T)). The filtrate in branch B was treated in the same way as branch B inwww.sunhealthmedical.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC 12V Power Relay for Smart House. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Medical Human Body Thermometer(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Greenhouse Fixing Film Profile Lock Channel and Spring Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Supplier Granular Coal Based Activated Carbon Filter Meida.Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Droshows the peptides separations after enzymatic hydrolysis.Glass Cutting Table/CNC Glass Cutting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPre-Seasoned Cast Iron Mini Oval Casserole Pot, but not shown inMicro Small Plastic Product Injection Molding Making Machine,12 Bottles Portable Home Ues Thermoelectric Wine Chiller, and also free amino acids (Whitaker et al., 1963).Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile,1996).

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CONCLUSIONS

Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector) for the financial support.

REFERENCES

High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Paulownia Wood Board Wood Drying Kilns for Sale]

Brown, E. M; Thompson, C .J.,Hanwha SMT Chip Shooter LED Pick and Place Machine, M.M.(1994),ACP Aluminium Cmposite Panel Cladding Extrusions.The Journal of the American Leather Chemists Association (JALCA)89(7)[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. Analytical Biochemistry212,436-445[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Custom Mens Heated Down Jacket. Biotechnology letters 14,6, 531-534[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non Standard U Channel Steel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier Granular Coal Based Activated Carbon Filter Meida]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zigzag Brochure Holder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction]

Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light]

Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit]

Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Marine Mooring Hardware, Anchor Chain, Part I.Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery]

Whitaker,J.R.(1963),Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Analytical Chemistry35(12), 1950-1953[High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)Author for correspondence