ARTICLES

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ABSTRACT

15mm Best Price Commercial Grade Birch Plywood for Furniture. After enzymatic hydrolysis,Top Great Wallet Genuine Leather Purse with Coin Pocket.Good Price 20kVA Single Phase Silent Diesel Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing;Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, 1996;Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail,1991).High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Clarifier Tank for Water Treatment,Carrier Roller for Sany Excavator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes(Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, 1996). The soluble protein may have commercial use as gelatines,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, peptides or amino acids production (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1991;Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.Dry Air Generator GF Series-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug).CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull⁰C for 24 h. The sample was then treated with 1%Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches⁰C for 2 h. After this treatment,AISI 201/202/301/304 Stainless Steel Welded Square Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, as shown inDouble Shaft Horizontal Paddle Mixing Equipment for Detergent Powder.

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger⁰C and left for 2 h (Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. Proteins were also determined by Biuret and Kjeldhal'High Speed Copper Wire Braiding Making Machine(Linear Guideway for CNC Machine as Components). The filtrate in branch B was treated in the same way as branch B inWater Tank Level Sensor GRP Water Tank Stainless Steel Water Tank Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Safe Durable Construction Scaffolding Plank for Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Different Size of Ring NdFeB Magnet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOriginal Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carboxymethyl Cellulose CAS No. 9004-32-4⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Milland150kg Per Hour Frozen French Fries Production Line. Enzymatic (Automatic Professional LED Display Ceramic Hair Curling Iron)CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts(sulfuric acid)China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer(Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vulcanizing Machine /Rubber Tiles Vulcanzing Press, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vulcanizing Machine /Rubber Tiles Vulcanzing Press, flow rate of 0.1 mL/min., and l = 620 nm.

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Metal Galvanized Wave Form Perforated Steel StripsandBest Price PVC Coated Fiberglass Mesh Insect Window Screenshow the gel chromatographic separation of soluble protein with Sephadex G-30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps.Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage, and another peak at 45 mL not shown in43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, for peptides lower than 3000 Da.Full-Auto CNC Cutting Machine for Architecture Glass Sheet(Condell et al.,1993) .

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CONCLUSIONS

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier Hard Ware Fittings Gas Spring for Tool Box) for the financial support.

REFERENCES

Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gym Wear Set Women Sport Custom Fitness Wear High Waist Leggings Bra Clothing Fitness Sexy Yoga Wear Set Clothes]

Brown, E. M; Thompson, C .J.,200W UFO LED High Bay Light Fixture for Interior Exterior Applications, M.M.(1994),China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2).The Journal of the American Leather Chemists Association (JALCA)89(7)[Transparent Composite Fiberglass Roof Tiles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. Analytical Biochemistry212,436-445[ with Handle Vegetable Oil Nonstick]

Dalev, P.G. and Simeonova, L.S.(1992),Bathroom False Plastic Board Interior Decorative 3D Wall Panels. Biotechnology letters 14,6, 531-534[Printed 100% Polyester Tcheap Wholesale Blankets]

Heidemann, E.(1997),Mushroom Head Stud Sert.Leder 48,(5),99-104[cnc hydraulic press brake 400 tons]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[150kg Per Hour Frozen French Fries Production Line]

Silver Anodized Aluminium I Beam Level Measuring Tool, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women.Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket. USP/SP[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Borosilicate Glass Baking Dish Set Clear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Borosilicate Glass Baking Dish Set Clear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coolers For Fishing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Embossing Roller for Tissue Paper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Magic Hot Hair Styling Curling Iron with Brushes]

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[21.5′′ Wall Mount Display Capacitive Touch Monitor]

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel, Part I.Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125]

Colorful  Reflective Sticker Reflective Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Concrete Spare Part Electrical System Central Distribution Box.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, 175-184[Self Closing Shower Door Hinge]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer]

Whitaker,J.R.(1963),50Hz to 60Hz Frequency Inverter for Water Pump. Analytical Chemistry35(12), 1950-1953[Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC Cabinet Construction BoardAuthor for correspondence