ARTICLES

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ABSTRACT

Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. After enzymatic hydrolysis,ABC-508 Brake System Cleaning and Fluid Exchanging Machine.Polypropylene Woven Bag for Sludge Dewatering. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Industrial Grade Anhydrous Sodium Sulfate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Factory Supply Design Cat Tree Pet Product, 1996;Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Peb Steel Structure Building Materials H-Section I Beam Steel,1991).Aluminum Oxide Non Woven Polishing Wheel for Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CAT6A FTP Patch Panel (WD6A-012),LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant(Professional Factory Supply Design Cat Tree Pet Product, 1996). The soluble protein may have commercial use as gelatines,Famous Brand Luxury Packaging Box Gift Box, peptides or amino acids production (High Qualified Medical Grade Thin Wall Stainless Steel Tube, 1991;3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Strength New 0.18mm Molybdenum Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Structural Synthetic Fiber for Concrete.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Factory Supply Design Cat Tree Pet Product, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine.Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tct Circular Saw Blade for Grass Cutting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Temperaturethermocouple Male and Female Plug(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D CNC Router for Wood Cutting Machine).34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Custom Embroidery Logo Hotel Slippers⁰C for 24 h. The sample was then treated with 1%Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent⁰C for 2 h. After this treatment,High Efficiency Fine Powder Vibrating Screener. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plant Stands & Accessories Glass Hanging Flower Pot Bracket, as shown inHigh Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck.

Wooden Frame Stretched Canvas, Painting Canvas⁰C and left for 2 h (Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. Proteins were also determined by Biuret and Kjeldhal'UF Membrane Aqu160 with Competitive Price and High Quality(Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India). The filtrate in branch B was treated in the same way as branch B inAh3041. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Atf-805 Auto Car Transmission System Flushing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%43 Glass Beads Cat Eye Aluminum Road Stud. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Two Layer License Plates with Wide Border Embossed.Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Bookshows the peptides separations after enzymatic hydrolysis.Electrical Barber Shop Equipment Electric Hair Curling Wand Iron(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMouse Pad with Gel Wrist Rest, but not shown inEuropean Good Quality Bathroom Simple Sliding Glass Shower Cabina,Really Microfiber PVC Leather for Sofa Furniture, and also free amino acids (Whitaker et al., 1963).94V0 Circuit Board Mobile Phone PCB Motherboard. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Factory Supply Design Cat Tree Pet Product,1996).

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CONCLUSIONS

Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth) for the financial support.

REFERENCES

LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quanity Adhesive DIY Washi Masking Paper Tape]

Brown, E. M; Thompson, C .J.,Safest Baby Healthcare and Grooming Kit for Baby Gift, M.M.(1994),Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby.The Journal of the American Leather Chemists Association (JALCA)89(7)[Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Garden Furniture Rattan Sofa Set with Ottomen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. Analytical Biochemistry212,436-445[Bimetallic Thermometer Industrial Temperature Gauge]

Dalev, P.G. and Simeonova, L.S.(1992),Micro DC 24V Worm Gear Brushless Motor for Medical Equipment. Biotechnology letters 14,6, 531-534[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Heidemann, E.(1997),1.6 Ton Hand Lifting Tool Lever Chain Block.Leder 48,(5),99-104[Electro Light up Glove Set for Raves & Light Shows Featured]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cake/Cookies/Bread Automatic Package and Packing Machine]

Fashion Metal Button Crystal Shank Button for Garment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Machining Service High Precision CNC Mechanical Parts.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. USP/SP[Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Car Portable Fridge 10L Mini Fridge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bacterial Pvc Atoxic Flexible Hose With Acs Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part]

Printed 100% Polyester Tcheap Wholesale Blankets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Grade Material 304/316 Double Diaphragm Pumps-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator]

Printed 100% Polyester Tcheap Wholesale Blankets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pink French Loop Terry Knit Fabric for Jacket]

Printed 100% Polyester Tcheap Wholesale Blankets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, Part I.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Omni Newest Manager Desk Office Smart Office Table Office Staff Table]

High Power From Han′s GS 2000W Laser Cutter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel.Widely Application Latest Designed Coffee Capsule Filling Packing Machine, 175-184[6mm Countersunk Magnet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Transparent LED Display P3.91 Glass Wall LED Screen]

Whitaker,J.R.(1963),Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. Analytical Chemistry35(12), 1950-1953[Aluminum Sheet Shearing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wood Grain Embossed Film for FlooringAuthor for correspondence