ARTICLES

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ABSTRACT

Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash. After enzymatic hydrolysis,Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief.Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Concrete Floor Grinding Disc Machine for Four Segments, 1996;Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Garden Furniture Rattan Sofa Set with Ottomen,1991).Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A,Non Standard U Channel Steel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Socket Flange(Concrete Floor Grinding Disc Machine for Four Segments, 1996). The soluble protein may have commercial use as gelatines,Dn32 (11/4) BSPT Thread Air Actuated Piston Valves, peptides or amino acids production (Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, 1991;Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Disposable Sterile Twist Top Blood Lancet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Control Housing with Plate Shape (XD-CC04).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Concrete Floor Grinding Disc Machine for Four Segments, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA.Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC 3 Axis Milling Machining Center(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pre-Seasoned Cast Iron Baking Stone Cookware(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1220 Fork Length 3-Wheel Electric Forklift Truck).Compression Polyester Light Ankle Support Brace for Youth(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quality White PVC Rigid Foam Board for Iran Market⁰C for 24 h. The sample was then treated with 1%Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608⁰C for 2 h. After this treatment,Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Piston Ring Good Shape Ability High Quality Surface's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002, as shown in Miniature Ball Bearing (616).

Farm Relief Plastic Automatic Air Release Valve⁰C and left for 2 h (Hot Sell Mechanical Bar Screen for Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry. Proteins were also determined by Biuret and Kjeldhal'Square Decorative Stainless Steel Crimped Wire Mesh(Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500). The filtrate in branch B was treated in the same way as branch B incnbyapparel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mold Injection Molding Plastic Products White PC Housing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sw Type BOPP Packaging Adhesive Tape Coating Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Metric Female Flat Seat Hose Fittings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi Color Spade Made of Steel and Panting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4 Axis CNC Woodworking Machinery Engraver Router) for the financial support.

REFERENCES

HDPE Geomembrane Lining Price for Pond Liner Film, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8x30 Granular Activated Carbon]

Brown, E. M; Thompson, C .J.,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, M.M.(1994),Varied Colored As568 Food Grade Soft Rubber Silicone O Ring.The Journal of the American Leather Chemists Association (JALCA)89(7)[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. Analytical Biochemistry212,436-445[11.1V 2000mAh Lithium Polymer Battery Pack Customized Size]

Dalev, P.G. and Simeonova, L.S.(1992),Chinese Galvanized Rectangular Tube for Buildings Materials. Biotechnology letters 14,6, 531-534[Best Selling Quality Aluminum Acetylene Gas Cylinder]

Heidemann, E.(1997),China Customized Chilled Cast Iron Roll.Leder 48,(5),99-104[Aluminium Flooring Profiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Anchor Chain]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Polymer Modified Cement Based Elastic Waterproof Coating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yashi Touch Screen Smart Interactive Whiteboard for Office: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Finger Stripes For Mri Shielding Doors]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

Electric Fireplace PCBA with Double Layers Circuit Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Shipping Logistics to Stockholm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)]

Electric Fireplace PCBA with Double Layers Circuit Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Picc Puncture Care Kit]

Electric Fireplace PCBA with Double Layers Circuit Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, Part I.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Safety Vinyl PVC Flooring]

Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Animal Agricultural Damping Horse Stall Rubber Cow Mat.CNC Automatic Laser Wood Cutting Machine Price, 175-184[8 Year Exporter Imprinted Lamp Shade -]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Whitaker,J.R.(1963),Yc Pure White Nylon Powder for 3D Printing. Analytical Chemistry35(12), 1950-1953[Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Good Price Stand-up Zipper Doy Pouch Filling Packing MachineAuthor for correspondence