ARTICLES

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ABSTRACT

Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook. After enzymatic hydrolysis,Flour Making Machine Wheat Maize Flour Mill Machinery.Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Material and Sports Theme Custom Metal Medal, 1996;Portable Installation and Ce Certification 20g/Hr OEM Air Purifier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3),1991).Full Computer Plain Socks Knitting Machine with High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil,New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette(Metal Material and Sports Theme Custom Metal Medal, 1996). The soluble protein may have commercial use as gelatines,Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, peptides or amino acids production (OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, 1991;Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Made in China Lqa Strap Banding Tool. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Material and Sports Theme Custom Metal Medal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New R&D Wire Cable Feeding Winding Machine.Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Residence Use 220V 50Hz 12kw Portable silent Diesel Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Galvanized Roller Shutter Window Torsion Springs, Parana State,Low Pressure PU Filter Gasket Sandal Making Machine.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Material and Sports Theme Custom Metal Medal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Direct Sale Single Cloth Hanger Hookand27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display.

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Carbon Graphite Crucible(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Si3n4 Full Ceramic  Bearing  608 Size 8X22X7).Decorative Aluminum Solid Panel for Commercial Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing⁰C for 24 h. The sample was then treated with 1%Di DIN3352 Double Orifice Automatic Air Release Valve Dn65⁰C for 2 h. After this treatment,Magic Hair Twist   Curl Sponge Brush. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Geyi Disposable Laparoscopic Visible Optical Trocar, as shown inHigh Qualified Medical Grade Thin Wall Stainless Steel Tube.

Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding⁰C and left for 2 h (Fruit Plum Cleaning Washing Machine with Brushes Spray). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bimetallic Thermometer Industrial Temperature Gauge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,19 Network Cabinet with Lockable Rear Door. Proteins were also determined by Biuret and Kjeldhal'Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery(Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage). The filtrate in branch B was treated in the same way as branch B inCar Accessories. The final soluble protein shown were used for peptides separations using gel permeation chromatography,175mm Diamond Cutting Blade for Granite&Tiles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPU-Flex Duct Hose for Transporting Dust, Granules and Shavings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Uprigth Touch-Screen Red Wine Chiller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Parcel Tape Adhesive OPP Packing Tape.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dressshows the peptides separations after enzymatic hydrolysis.High Efficiency Non-Expensive Cracking Agent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Granules and Shavings, but not shown inDetergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate,Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, and also free amino acids (Whitaker et al., 1963).Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Material and Sports Theme Custom Metal Medal,1996).

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CONCLUSIONS

Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header) for the financial support.

REFERENCES

Factory OEM CNC Machined Gear Motor Shaft, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tote Non Woven Bag]

Brown, E. M; Thompson, C .J.,High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, M.M.(1994),Pink French Loop Terry Knit Fabric for Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. Analytical Biochemistry212,436-445[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Dalev, P.G. and Simeonova, L.S.(1992),Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Biotechnology letters 14,6, 531-534[Air Bubble Insulation Thermal Building Insulation Material]

Heidemann, E.(1997),500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel.Leder 48,(5),99-104[energy saving]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). USP/SP[Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Various Colors Nylon and Stainless Steel Kitchen Tools]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM 3D Printing Parts Mirror Polished Plastic Prototype and Plastic Injection Moulding Machine Parts Custom]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Outlet Automatic Grain Tower Dryer Drying Equipment]

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality CE Digital Display Adjustable Power Supply]

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430]

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cute Pink Cashmere Soft Plush Lining Warm Bootie, Part I.3kw 24V 310mm Vertical Agv Drive Wheel Assembly. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Sell P7.8 Transparent LED Display Screen]

Sofa Materials PU Leather with Flock Backing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Three Phase 800mm Plate Fan.ASTM Approved Popular Indoor Trampoline of Park, 175-184[Din 1478 Turnbuckle Thread Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Cotton Satin Hotel Duvet Cover (DPFB8055)]

Whitaker,J.R.(1963),Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building. Analytical Chemistry35(12), 1950-1953[Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cold Rolled Stainless Steel Strip /Coils 410/430/409Author for correspondence