ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Enamel Cast Iron Mini Casserole Cast Iron Cocotte. After enzymatic hydrolysis,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part.Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC System Air Duct Line5 Make Machine for HVAC, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Titanium Dioxide Anatase for Paper Making, 1996;40A C Electronic Air Circuit Breaker with Dz47-4p., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe Thermostat with Sensor Ce Certificate,1991).Walking Long Distance Surveying Instrument Meter Measuring Wheel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Supplier of PVC Profile,Tianjin Manufacturer PTFE Coated Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard(Titanium Dioxide Anatase for Paper Making, 1996). The soluble protein may have commercial use as gelatines,Wholesale Prices of 20W 40W 60W Solar Street Lights, peptides or amino acids production (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1991;Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ALS Stage 5W RGB Animation Laser Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Titanium Dioxide Anatase for Paper Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch.Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet Anti-Static Needled Mat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Dz/Bt-80h Multifunctional Full Automatic Cartoning MachineAlcalase2.5 L DX,OEM Sheet Metal Fabrication for Aluminum Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hwf Tsp Core Bit for Geotechnical Drilling); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Price of Automatic Sludge Dewatering Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, Parana State,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.Worm Gear Operation Wafer Lined F3 Butterfly Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Titanium Dioxide Anatase for Paper Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODFand48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System.

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Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding).Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Positive Drive Turn Belt for Spiral Freezing Equipment⁰C for 24 h. The sample was then treated with 1%Chinese Herb Medicine Angelica Root Extract⁰C for 2 h. After this treatment,S Series China Generator Helical-Worm Crushers Reduction Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Original Testo 174t Mini Temperature Data Logger No. 0572 1560, as shown inFashion Shoulder Backpack Handbag School Bag.

Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water⁰C and left for 2 h (Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Proteins were also determined by Biuret and Kjeldhal'Whole Set Gas Spring Strut for Furniture(Security Shopping Trolley Baby Seat for Supermarket Cart). The filtrate in branch B was treated in the same way as branch B inRapid Delivery for Hub Bolt And Nut Oem - Flat washer-silvery-black - Jiangyu . The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dia. 5.5mm Bonding ND: YAG Laser Rod Lens.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining Turning Anodized Aluminum Worm Gear. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Solar Panel Solar Cell Photovoltaic 300W Hot Sell(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Planter Moving Round Planter Saucer Casters Plant Trolley (M)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Water Meter Spare Parts Rubber Rings for Sealing.Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Meshshows the peptides separations after enzymatic hydrolysis.Stainless Steel HK Series Needle Roller Bearing HK0408(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRechargeable Battery Operated Cooling Folding Electric Fan, but not shown inTelescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop,Road Construction Fabric Non-Woven Geotextile 200GSM, and also free amino acids (Whitaker et al., 1963).High Polymer Modified Cement Based Elastic Waterproof Coating. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Titanium Dioxide Anatase for Paper Making,1996).

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Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, V_e, and K_avcalculated as discussed previously.Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl,FTTH Fiber Optic Fusion Splicing Toolkit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fused Quartz Powder 325mesh 500mesh 1250mesh) (Reis et al.,1989;Sinicline Custom Brand with Plastic Pouch Hangtag for Hats.,1991;40A C Electronic Air Circuit Breaker with Dz47-4p.,1991).High Speed Cable Wire Winding Braiding Machine(1992), were lower, except for cystine and tyrosine,22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill.

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CONCLUSIONS

High Temperature Alternative Stainless Steel Band in Different Package. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hwf Tsp Core Bit for Geotechnical Drilling) for the financial support.

REFERENCES

Water Cooled Low Temperature Screw Chiller for Industrial Workshop, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rocker Switch Wiring Harness]

Brown, E. M; Thompson, C .J.,Home Machine 2019 New Commercial Treadmill Gym Equipment, M.M.(1994),Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[ X Ray Room Door]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Residential Reverse Osmosis 0.0001um Water Filter Water Filtration. Analytical Biochemistry212,436-445[Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle. Biotechnology letters 14,6, 531-534[Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing]

Heidemann, E.(1997),China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses.Leder 48,(5),99-104[Roller And Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine]

Chicken Cage Mesh Hexagonal Wire Mesh Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Folding Round Picnic Caravan Table RV Picnic Table Camping Table.Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits. USP/SP[Flour Making Machine Wheat Maize Flour Mill Machinery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3HP Industrial Cooling System Water Cooled Chiller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[80W All in One Outdoor LED Street Light Solar Lamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[as-Mc Classic European Style Wall Papers Home Decor Embossed 3D Damask Wallpaper Roll Bedroom Living Room Sofa TV Background]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Veneer Peeling and Clipping Combined Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Explosion-Proof Housing Sealing Dispensing Machine]

OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Easy Operational Cup Cake Filling Machine]

OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone, Part I.Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall.Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker, 175-184[Gas Soup Kettle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tomato Bubble and High Pressure Washing Machine]

Whitaker,J.R.(1963),Corrugated Board Production Line. Analytical Chemistry35(12), 1950-1953[Disposable PS Foam Fast Food Box Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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