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ABSTRACT

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. After enzymatic hydrolysis,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration;Southeast Asia Healthy Soft Integrated Latex Foam Mattress; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Water Filter System Lamella Clarifier for River Water Treatment System, 1996;Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cabinet Shelf Support Pin Furniture Hardware Fittings,1991).Reasonable Price High Quality Nylon Cable Gland. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan,Disposable Anesthesia Breathing Circuit Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type(Water Filter System Lamella Clarifier for River Water Treatment System, 1996). The soluble protein may have commercial use as gelatines,Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element, peptides or amino acids production (New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, 1991;Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Counter-Clip Fluorine-Lined Butterfly Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC Brushless Motor for CNC Machine with ISO9001.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Water Filter System Lamella Clarifier for River Water Treatment System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Road Safety Warning Barricade LED Solar Powered Blinking Light.Manufacturer Customized Plastic Injection Molding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K), Parana State,White Color Enamel Bowls with High Quality.Custom Aluminum Cover Frame Half Aluminum Cover Profile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Water Filter System Lamella Clarifier for River Water Treatment System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Polymer Modified Cement Based Elastic Waterproof CoatingandSs 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.

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Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)).DC24V 36W Solar Fan Motor PCBA Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Monocular Hunting Wargame Mini Scope Digital Night Vision⁰C for 24 h. The sample was then treated with 1%Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine⁰C for 2 h. After this treatment,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Italy Design Good Quality Hydraulic Motorcycle Lift Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, as shown inPicc Puncture Care Kit.

Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids⁰C and left for 2 h (Hot Sale Safety Vinyl PVC Flooring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Portable Telescopic Straw Metal Drinking Straw with Brush Case. Proteins were also determined by Biuret and Kjeldhal'Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware(Tianjin Manufacturer PTFE Coated Butterfly Valve). The filtrate in branch B was treated in the same way as branch B inCustom 16X4 Character 8pin Display I2c Module 16*4 Monochrome LCD Display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Etching Rear Trunk Sill Plates for Hyundai Tucson.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLab Equipment Sr Paper Pulp Beating Freeness Test Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light) for the financial support.

REFERENCES

Pure Sine Wave Power Frequency Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2018 Industrial Gases Liquids Production Plant]

Brown, E. M; Thompson, C .J.,Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, M.M.(1994),Colorful 100% Cotton Hotel Face Towels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Three Phase 800mm Plate Fan]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S). Analytical Biochemistry212,436-445[Brushed Anodizing Aluminum Extrusion Profile for LED]

Dalev, P.G. and Simeonova, L.S.(1992),33kv Dry Type Resin Casted Distribution Transformer. Biotechnology letters 14,6, 531-534[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Spare Parts for Caterpillar Construction Machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sinicline 2018 Hot Sell Custom Printing Thank You Cards: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Card Games For 2 People]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Black Red Open Net Spandex Nylon Sexy Lingerie, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Black Red Open Net Spandex Nylon Sexy Lingerie,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[36PCS LED Gold Matrix Stage Zoom Light]

Black Red Open Net Spandex Nylon Sexy Lingerie, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, Part I.LED Emergency Exit Lights. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glow Powder for Luminous Coating]

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine, 175-184[Best Price of China Factory Welding Ss Stainless Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

Whitaker,J.R.(1963),Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY. Analytical Chemistry35(12), 1950-1953[Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Welding StructureAuthor for correspondence