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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Carboxy Terminal Collagen Crosslinks Elisa Kits. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Various Colors Nylon and Stainless Steel Kitchen Tools

ABSTRACT

355nm UV Laser Marking Equipment for Crystal Materials. After enzymatic hydrolysis,Metal Separating Gold Brass Zippers for Sale.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Refrigeration Digital Thermometer;BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, 1996;Two Roll Rubber & Plastic Mixing Mills Tire Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes,1991).Hot Sale 25kw Grid Tied Home Solar Panel System Kit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Car Portable Fridge 10L Mini Fridge,Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Small Garden Excavator Digging Work(79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, 1996). The soluble protein may have commercial use as gelatines,Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver, peptides or amino acids production (Offshore Drilling Platform Air Winch for Marine Oilfields, 1991;Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Industry Electric Transport Vehicle on Rails., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manual Interlocking Brick Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing.Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Eye Steam Mask).Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Foot Pedal Hydraulic Pump⁰C for 24 h. The sample was then treated with 1%860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag⁰C for 2 h. After this treatment,Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CH Series Pid Intelligent Temperature Controller CH402's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory, as shown inCarrier Roller for Sany Excavator.

Disposable Precision Infusion Set with Filter and Needle for Single Use⁰C and left for 2 h (Hot DIP Galvanized G2150 D Ring Shackle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Price Sweep Frequency Response Analyzer for Power Transformer Test. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Mechanic Parts for Lock Accessories with MIM Process(Aluminum Oxide Non Woven Polishing Wheel for Machine). The filtrate in branch B was treated in the same way as branch B inFree Angle Hinged Elbow Immoblizaiton Fixing Support Splint Brace Manufacuture. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mining Machinery Sand Vibrating Sieve for River Sand. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStraight-Through Medium Flange Hard-Sealed Z41h Gate Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Palet Coil Nail Welding Machine/Nail Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality MPO Singlemode Duplex Fiber Optic Adapter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Security Shopping Trolley Baby Seat for Supermarket Cart) for the financial support.

REFERENCES

Snsc Fd30 Side Shift New Diesel Forklift 3ton, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[MDF Coaster]

Brown, E. M; Thompson, C .J.,Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), M.M.(1994),Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type.The Journal of the American Leather Chemists Association (JALCA)89(7)[Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laptop Bag College Men Backpack Leather Back Pack Bags. Analytical Biochemistry212,436-445[Dia. 5.5mm Bonding ND: YAG Laser Rod Lens]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Dog Grooming Rake Dematting Tool Shedding Comb. Biotechnology letters 14,6, 531-534[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

Heidemann, E.(1997),Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.Leder 48,(5),99-104[608zz Bearing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Marble Stair Tread]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

Automatic Food Metal Detector Machine for Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Multi-Functional Backpack Manual 52cc Brush Cutter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flat Pack Temperary Container House 20FT 40FT]

Automatic Food Metal Detector Machine for Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Engieered Veneer Faced Commericial Plywood]

Automatic Food Metal Detector Machine for Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, Part I.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Whitaker,J.R.(1963),PAC /Poly Aluminium Chloride for Water Treatment. Analytical Chemistry35(12), 1950-1953[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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