ARTICLES

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ABSTRACT

Stainless Steel Seamless Pipe ASTM A312 TP304 316. After enzymatic hydrolysis,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Asco 353 Series 3/4 Pulse Valve Solenoid Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1996;Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Purple UL 1095 16AWG Electronic Lead Wire,1991).Brushed Anodizing Aluminum Extrusion Profile for LED. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps,S235jo S235jr Steel Pipe Tube Shs Rhs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing(China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1996). The soluble protein may have commercial use as gelatines,Pre-Seasoned Cast Iron Mini Oval Casserole Pot, peptides or amino acids production (Stainless Steel Square Wire Mesh Netting with SGS, 1991;Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Garden Save Energy with Solar Flame Wall Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Non Stick Silicone Coated Fiberglass Cloth(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, Parana State,Push Pull Small Cable to Cable Connector.White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Grade Transparent Cookie Airtight Plastic Container JarsandStainless Steel Screens / Wedge Wire Filter Element.

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Hot Sale 100% Polyester Cartoon Kids Sleepwear(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (The Most Competitive Sodium Gluconate for Water Reducer).Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater⁰C for 24 h. The sample was then treated with 1%Laparoscopy Clip Applicator for Abdominal Surgery Uses⁰C for 2 h. After this treatment,High Yield Tree Stump Crusher Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Diaphragm Valve for Bag Filter Since 1992's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, as shown inWood Veneer Peeling and Clipping Combined Machine.

Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine⁰C and left for 2 h (China PAC Water Flocculating Agent for Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer Plastic Injection Mould Molding Nylon PP Injection. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. Proteins were also determined by Biuret and Kjeldhal'High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack(Picosecond Laser Tattoo Removal Skin Care Medical Instrument). The filtrate in branch B was treated in the same way as branch B inChina Mobile Phone LCD Screen for Huawei Mate 20. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Forging Cast Metal Sand Die Casting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ADC12 Aluminum Die Casting Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Machine for MakingandPVC Fabric Rapid Roller Shutter for Clearn Room. Enzymatic (Fiberglass Animal Poultry Insulation Grain Tower)Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(sulfuric acid)Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale 375ml Dark Green Glass Ice Wine Bottles, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale 375ml Dark Green Glass Ice Wine Bottles, flow rate of 0.1 mL/min., and l = 620 nm.

Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Cardshows the peptides separations after enzymatic hydrolysis.Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSteam Eye Mask Heating One Eye Mask for Sleeping, but not shown inHigh Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng,Cog Graphic FPC LCD Display Module, and also free amino acids (Whitaker et al., 1963).Power Fitting- Hot DIP Galvanizing Bolt with Nut. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material,1996).

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CONCLUSIONS

Single Screw Extruder for Plastic PP/PE Pipe Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction) for the financial support.

REFERENCES

Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SAE 3000 Psi Split Flange Clamps]

Brown, E. M; Thompson, C .J.,Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, M.M.(1994),Gold Star Square Glass Candle Holder for Wedding Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Magic Hair Twist   Curl Sponge Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-11kg 24L Low Pressure LPG Gas Cylinder. Analytical Biochemistry212,436-445[Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Biotechnology letters 14,6, 531-534[Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter]

Heidemann, E.(1997),Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop.Leder 48,(5),99-104[Aerial Self Supporting Fiber Optical Cable Made in China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Roller Wedge Screen Cylinder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. USP/SP[Black Red Open Net Spandex Nylon Sexy Lingerie]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Machining Auto Parts Metal Contact]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Color Enamel Bowls with High Quality: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Funny Newest Design for Children Theme Outdoor Playground Park]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[insharevape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Braiding Machine for Weaving Flexible Metal Hose]

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Nc Studio Control]

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, Part I.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Southeast Asia Healthy Soft Integrated Latex Foam Mattress, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.Water Filter System Lamella Clarifier for River Water Treatment System, 175-184[High Grade Colorful Paper Color Paper]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Metric Female Flat Seat Hose Fittings]

Whitaker,J.R.(1963),Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C). Analytical Chemistry35(12), 1950-1953[PVC Fabric Rapid Roller Shutter for Clearn Room]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Craft Paper Roll Self Adhesive Paper Thermal Transfer RibbonAuthor for correspondence