ARTICLES

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ABSTRACT

High Borosilicate Glass Baking Dish Set Clear. After enzymatic hydrolysis,G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.Embossing Roller for Tissue Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful  Reflective Sticker Reflective Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Concrete Spare Part Electrical System Central Distribution Box, 1996;Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (50Hz to 60Hz Frequency Inverter for Water Pump,1991).Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325),4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator(Concrete Spare Part Electrical System Central Distribution Box, 1996). The soluble protein may have commercial use as gelatines,PVC Cabinet Construction Board, White 18mm PVC Foam Board, peptides or amino acids production (2019 New Portable Mini Dental X-ray Machine, 1991;Ss Reduced Port 2000wog 1PC Screwed Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Prefab Steel Building for Garment Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Concrete Spare Part Electrical System Central Distribution Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cable Tray with ′t′ Slots.10 Gauge Galvanized Welded Wire Mesh for Construction Using-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Factory Inline Hockey Skate Puck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAccept OEM New Products Team Various Colors Gold Ribbon(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Supplier Granular Coal Based Activated Carbon Filter Meida. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable PS Foam Fast Food Box Making Machine) for the financial support.

REFERENCES

Glass Cutting Table/CNC Glass Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Industrial Food Cooking Pot]

Brown, E. M; Thompson, C .J.,12 Bottles Portable Home Ues Thermoelectric Wine Chiller, M.M.(1994),Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). Analytical Biochemistry212,436-445[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

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Heidemann, E.(1997),Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening.Leder 48,(5),99-104[Cost Septic Tank]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Mushroom Head Stud Sert, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle.Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe. USP/SP[ Shampoo Mascara Titling Mixing and Emulsifying Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pure Sine Wave Power Frequency Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Inductor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Female Robot Costume]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce]

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Bar Sink]

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Extruded Alloy Aluminum Tube 1060 1070 3A21]

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, Part I.High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hairs Comb for Pet Dog Cats]

Hanwha SMT Chip Shooter LED Pick and Place Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ACP Aluminium Cmposite Panel Cladding Extrusions.New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, 175-184[Instant L-Isoleucine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN 7337 Aluminium Core Pulling Stainless Steel Rivet]

Whitaker,J.R.(1963),Wholesale Custom Mens Heated Down Jacket. Analytical Chemistry35(12), 1950-1953[Popular Lovely Animal Design Inflatable Slide for Kids]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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