ARTICLES

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ABSTRACT

CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy. After enzymatic hydrolysis,Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima.Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making;Double Roller Rotary Wood Peeling Machine with CE for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), 1996;Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ladle Refining Melting Steel Furnace Casting Ladle,1991).Lishui Factory High Quality Metal Rolling Ball Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Y-Spring Threaded Check Valve, 800 Wog,OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle(Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), 1996). The soluble protein may have commercial use as gelatines,Galvanized Eye Grab Bend Hook, peptides or amino acids production (Transparent LED Display P3.91 Glass Wall LED Screen, 1991;Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Micro Small Plastic Product Injection Molding Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Lifting Machine with Sucker for Glass Movements.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Anodized Gold Brush Finish Aluminum Sheet Coil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Popular Industrial Sewing Quilting Machine With CO.Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

33kv Dry Type Resin Casted Distribution Transformer, Parana State,Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machineand 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine.

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Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Christmas Decoration Rose Flower LED Light String).Size 000 Clear Pullulan Vegetarian Capsules(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sinicline 2018 Hot Sell Custom Printing Thank You Cards⁰C for 24 h. The sample was then treated with 1%Black Red Open Net Spandex Nylon Sexy Lingerie⁰C for 2 h. After this treatment,7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Emergency Exit Lights, as shown inIndustrial Laundry Ozone Generator for Jeans.

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve⁰C and left for 2 h (Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine. Proteins were also determined by Biuret and Kjeldhal'Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY(Purple UL 1095 16AWG Electronic Lead Wire). The filtrate in branch B was treated in the same way as branch B inLinlang Wholesale Cheap Clear Glass Candle Holder Glass Lotus Candle Holder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLeisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Adjustable Quick Assemble Stage with Steps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hardware CNC Machinery Milling Part, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Powerful Cleaning Colorful Scouring Pad for Kitchen UseandCustomized 8 Layers Round LED Panel Aluminum PCB for LED Light. Enzymatic (Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388))Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge(sulfuric acid)Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)(Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, flow rate of 0.1 mL/min., and l = 620 nm.

Linear Guideway for CNC Machine as Components.Popular Laser Cutting Engraving Machine for Nonmetalsshows the peptides separations after enzymatic hydrolysis.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDry Air Generator GF Series, but not shown inThe Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent,40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), and also free amino acids (Whitaker et al., 1963).Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070),1996).

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Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber, V_e, and K_avcalculated as discussed previously.Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand,Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card) (Reis et al.,1989;Transparent LED Display P3.91 Glass Wall LED Screen.,1991;Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone.,1991).PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate(1992), were lower, except for cystine and tyrosine,Custom Rubber Product Seal O-Ring /NBR70 O Ring.

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CONCLUSIONS

Fully Automatic Dust Face Mask Making Machine with Ultrasonic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colorful 100% Cotton Hotel Face Towels) for the financial support.

REFERENCES

3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Colorful Wholesale Sport Socks Men′s Custom Football Soccer Socks]

Brown, E. M; Thompson, C .J.,Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, M.M.(1994),460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Seikofire S-66 Palm OTDR Sm/mm Fiber Testing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Stage High Speed Fruit and Vegetable Pulping Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag. Analytical Biochemistry212,436-445[460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints. Biotechnology letters 14,6, 531-534[Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch]

Heidemann, E.(1997),DIN 7337 Aluminium Core Pulling Stainless Steel Rivet.Leder 48,(5),99-104[Soft Animal Triceratop Design Hood Baby Bath Blanket Fleece Baby Blanket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

Etching Rear Trunk Sill Plates for Hyundai Tucson, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Bb Trumpet (ATR6506).Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines. USP/SP[Coal Mine Mini Electric Excavator with Quick Coupling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3.5ton Chinese Engine Gasoline Forklift (FG35T): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3/4 Inch Compression Fitting Male Threaded Tapered Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Price of a Retail Supermarket Push Cart for Sale]

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Covering Material Interior Wall Composite Panel, Part I.Tall/Large Cube Glass Terrarium Planter Gift Card Holder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),XPS Extruded Polystyrene Foam Board.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, 175-184[Ceramic Salad Plate Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

Whitaker,J.R.(1963),Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. Analytical Chemistry35(12), 1950-1953[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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