ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Toilet Roll Tissue Wrapping Machine with Auto - Counting

ABSTRACT

Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine. After enzymatic hydrolysis,Orange Monofilament Fishing Tackle Nylon Fish Net.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;By6-8-200-L Automatic Doypack Premade Pouch Packing Machine;Diameter 1300mm Lifting Electro Magnet for Cast Iron; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Evaporator Finned Tube, 1996;Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine,1991).100%Polyester Spandex Hotel Chair Cover (DPH7022). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supply Perforated Metal Mesh,CNC Turning Center, CNC Horizontal Turning Milling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16(Evaporator Finned Tube, 1996). The soluble protein may have commercial use as gelatines,Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, peptides or amino acids production (Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, 1991;Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Wholesale Zinc Plated Carbon Steel Hex Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Evaporator Finned Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Cheese Making Machine Electric.Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100W Dimmable LED Corn Bulb with Meanwell Driver(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4-14 Manual Staple Gun with Staples for Hand Tools, Parana State,Toilet Roll Tissue Wrapping Machine with Auto - Counting.Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Evaporator Finned Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChinese Knuckle Boom Hydraulic Truck Mounted Crane for SaleandNew Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace.

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Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away).Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Biomass Drum Wood Chipper with Competitive Price⁰C for 24 h. The sample was then treated with 1%High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags⁰C for 2 h. After this treatment,Forged Steel Pressure Sealed Bonnet Globe Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Injection Moulding Machine Supplier / Factory /Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, as shown in14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.

15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable⁰C and left for 2 h (Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,80W All in One Outdoor LED Street Light Solar Lamp. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Safety Vinyl PVC Flooring(Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap). The filtrate in branch B was treated in the same way as branch B inPopular Hot Sale Chinese Approved Tracked 6.5HP Gas Power Mini Dumper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Horizental Stainless Steel Food Grade Milk Tank for Milking. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulti Parameter Small Digital Domestic Water Meter TDS Meter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sanhe FC-2 Poultry Air Vent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Picosecond Laser Tattoo Removal Skin Care Medical Instrument, pH 3.25., mobile phase B: buffer solution of 0.01 mol/XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Machining Non-Standard Hardware PartsandIgnition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. Enzymatic (Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart)SUS316 Hexagon Socket Set Screws with Cone Point, DIN914(sulfuric acid)Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(Evaporator Finned Tube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, flow rate of 0.1 mL/min., and l = 620 nm.

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC.Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauceshows the peptides separations after enzymatic hydrolysis.Explosion-Proof Housing Sealing Dispensing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, but not shown inFast Supplier Juice Sealing Film Wrapping Packing Machine Production Line,Tube Mills for Thin Wall Tubing, and also free amino acids (Whitaker et al., 1963).Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Evaporator Finned Tube,1996).

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CONCLUSIONS

Tcn Sex Toy Vending Machines for Sale S800-10. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hexagonal Wire Mesh Chicken Wire Mesh High Quality) for the financial support.

REFERENCES

Silver 8 Inch Double Thick Round Cake Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Candle Gift Tube Box Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,High Precision Machining Auto Parts Metal Contact, M.M.(1994),Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label.The Journal of the American Leather Chemists Association (JALCA)89(7)[L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Bearing with Housing Bearing / Pillow Block Bearing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler. Analytical Biochemistry212,436-445[Milling Precision Stainless Steel CNC Machinery Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Paper Sticky Lint Remover Roller/Pet Hair Roller. Biotechnology letters 14,6, 531-534[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Heidemann, E.(1997),Safe Durable Scaffolding Trapdoor Plank for Construction.Leder 48,(5),99-104[Filter Cartridge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2 Round Sash Pulley for Chain - Brass (PVD)]

Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.Various Sublimation Blank Home Supply Fabric Bath Towel etc. USP/SP[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[33kv Dry Type Resin Casted Distribution Transformer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 Strong Soft Power Bedroom Furniture 8 Inch Spring Mattress in Pillow Top Design]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Festival Ceramic Mug]

Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mint Flavor 50ml Home Foam Toothpaste]

Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Quality Bending Metal Mould with All Kinds Metal Materials, Part I.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fly]

Milling Precision Stainless Steel CNC Machinery Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails.Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, 175-184[Big Sheet Metal Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

Whitaker,J.R.(1963),Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. Analytical Chemistry35(12), 1950-1953[Cable Tray with ′t′ Slots]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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