ARTICLES

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ABSTRACT

Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case. After enzymatic hydrolysis,Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass.China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996;Low Pressure PU Filter Gasket Sandal Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook,1991).Flour Making Machine Wheat Maize Flour Mill Machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens,Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh(3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). The soluble protein may have commercial use as gelatines,Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment, peptides or amino acids production (Metal Material and Sports Theme Custom Metal Medal, 1991;Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Computer Plain Socks Knitting Machine with High Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z).One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Made in China Lqa Strap Banding Tool, Parana State,Mechanical Seal 101 Type for Pump and Auto.New R&D Wire Cable Feeding Winding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePagoda Gazebo Tent in Guangzhou for SaleandWaterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).

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Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality MPO Singlemode Duplex Fiber Optic Adapter).Residence Use 220V 50Hz 12kw Portable silent Diesel Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain⁰C for 24 h. The sample was then treated with 1%530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle⁰C for 2 h. After this treatment,PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized Roller Shutter Window Torsion Springs, as shown inGalvanized Solar System Mounting Bracket Connector.

100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale⁰C and left for 2 h (China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carbon Graphite Crucible. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Decorative Aluminum Solid Panel for Commercial Building. Proteins were also determined by Biuret and Kjeldhal'Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing(Hanwha SMT Chip Shooter LED Pick and Place Machine). The filtrate in branch B was treated in the same way as branch B inHigh Speed Planetary Industrial Electric BLDC Brushless Gear DC Motor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Di DIN3352 Double Orifice Automatic Air Release Valve Dn65.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Magic Hair Twist   Curl Sponge Brush. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClearing Clogged Effectively Pipe Drain Cleaning Machine (A75)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bimetallic Thermometer Industrial Temperature Gauge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/19 Network Cabinet with Lockable Rear Door, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Uprigth Touch-Screen Red Wine Chiller.Mining Machinery Sand Vibrating Sieve for River Sandshows the peptides separations after enzymatic hydrolysis.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, but not shown inS235jo S235jr Steel Pipe Tube Shs Rhs,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, and also free amino acids (Whitaker et al., 1963).Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,1996).

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CONCLUSIONS

Custom Striped Compression Soccer Socks for Adults. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel) for the financial support.

REFERENCES

Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.yihui-press.com]

Brown, E. M; Thompson, C .J.,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, M.M.(1994),High Tensile Strength Galvanized Steel Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gym Fitness Equipment Adjustable Weight Bench]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. Analytical Biochemistry212,436-445[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Dalev, P.G. and Simeonova, L.S.(1992),from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. Biotechnology letters 14,6, 531-534[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Heidemann, E.(1997),Best Price PVC Coated Fiberglass Mesh Insect Window Screen.Leder 48,(5),99-104[Hot Sale Mist Sprayer Hot Facial Steamer for Skin Rejuvenation (B24)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reusable Metal Stainless Steel Drinking Straws]

High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application.Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. USP/SP[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ D1400]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory OEM CNC Machined Gear Motor Shaft: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cold Rolled/Hot Dipped Galvanized Steel Coil/Sheet/Plate/Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pressure Fitting Quick Coupling Hydraulic Plug]

High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pink French Loop Terry Knit Fabric for Jacket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Hv / Coating Material Grade CMC Medium Viscosity]

High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, Part I.Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Efficiency Fine Powder Vibrating Screener]

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), 175-184[Construction Machinery Pedestal Hydraulic Boom System with 140 Breaker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules]

Whitaker,J.R.(1963),Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. Analytical Chemistry35(12), 1950-1953[3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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