ARTICLES

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ABSTRACT

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine. After enzymatic hydrolysis,Home and Hospital Electric Patient Lift.Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DC 12V Power Relay for Smart House, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, 1996;Medical Human Body Thermometer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.,1991).Greenhouse Fixing Film Profile Lock Channel and Spring Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products,Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc(2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, 1996). The soluble protein may have commercial use as gelatines,48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, peptides or amino acids production (Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 1991;Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Supplier Granular Coal Based Activated Carbon Filter Meida., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Cutting Table/CNC Glass Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12 Bottles Portable Home Ues Thermoelectric Wine Chiller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, Parana State,What′s Kind of Machine for Making Organic Fertilizer Granules.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA SpeakerandQJ-M Moving small manual brick making machine with low cost.

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Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Galvanized Pressure Locked Grating).High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hanwha SMT Chip Shooter LED Pick and Place Machine⁰C for 24 h. The sample was then treated with 1%ACP Aluminium Cmposite Panel Cladding Extrusions⁰C for 2 h. After this treatment,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Custom Mens Heated Down Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, as shown inProfessional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine.

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker⁰C and left for 2 h (Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Swim Shorts Grey Mens Board Shorts with Mesh Liner. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. Proteins were also determined by Biuret and Kjeldhal'Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(Various Types of Plastic Hooks Mould From Manufacturers). The filtrate in branch B was treated in the same way as branch B inIndoor/Outdoor Waterproof Pigtail Fiber Optic Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Marine Mooring Hardware, Anchor Chain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLaser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.QJ-M Moving small manual brick making machine with low cost⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accordshows the peptides separations after enzymatic hydrolysis.ABC-508 Brake System Cleaning and Fluid Exchanging Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, but not shown inHome Machine 2019 New Commercial Treadmill Gym Equipment,Polypropylene Woven Bag for Sludge Dewatering, and also free amino acids (Whitaker et al., 1963).China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment,1996).

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Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden PatternandHigh Pressure Braided Rubber Oil Hoseshow the gel chromatographic separation of soluble protein with Sephadex G-High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.High Quality Industrial Grade Anhydrous Sodium Sulfate, and another peak at 45 mL not shown in25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, for peptides lower than 3000 Da.Professional Factory Supply Design Cat Tree Pet Product(Condell et al.,1993) .

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Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), V_e, and K_avcalculated as discussed previously.Peb Steel Structure Building Materials H-Section I Beam Steel,Aluminum Oxide Non Woven Polishing Wheel for Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10 Gauge Galvanized Welded Wire Mesh for Construction Using) (Reis et al.,1989;Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder.,1991;Medical Human Body Thermometer.,1991).CAT6A FTP Patch Panel (WD6A-012)(1992), were lower, except for cystine and tyrosine,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.

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CONCLUSIONS

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe) for the financial support.

REFERENCES

Famous Brand Luxury Packaging Box Gift Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Construction Signs]

Brown, E. M; Thompson, C .J.,High Qualified Medical Grade Thin Wall Stainless Steel Tube, M.M.(1994),3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Strength New 0.18mm Molybdenum Wire. Analytical Biochemistry212,436-445[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Structural Synthetic Fiber for Concrete. Biotechnology letters 14,6, 531-534[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Heidemann, E.(1997),LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer.Leder 48,(5),99-104[Aerolite Broken Retro Luggage]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tct Circular Saw Blade for Grass Cutting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Supplier V Wedge Wire Johnson Water Well Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vaulting Block For Kids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Herb Medicine Angelica Root Extract]

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Picc Puncture Care Kit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine]

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, Part I.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Paper Sticky Lint Remover Roller/Pet Hair Roller]

34*25cm Office File Pocket Felt A4 Document Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PP Needlepunched Nonwoven Geotextile.High Temperaturethermocouple Male and Female Plug, 175-184[Brusher Making Line Supplier Best-Selling]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Whitaker,J.R.(1963),34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. Analytical Chemistry35(12), 1950-1953[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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